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Recombinant Listeriolysin O PEST minus

Description: Recombinant listeriolysin O without PEST sequence and with haemolytic activity. The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. PEST sequence is 19 amino acids peptide located at the protein NH 2-terminus, that targets the toxin for degradation. This motif is essential for bacterial virulence.


Product type: Recombinant protein biologically active


Peptide: Listeriolysin O amino acidic sequence from L. monocytogenes without PEST sequence – Full-length protein accession number: AF253320.1


Expression system: Escherichia coli


Purity: >90% pure estimated by SDS PAGE (EU Ph. 5.0 § 2.5.31)


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Price:
€ 345,00

Info

Form: Liquid

Storage buffer: 50 mM NaH 2PO4, 0.5 M NaCl, 2.7 mM KCl, 1 mM EDTA, 1 mM DTT, 5%(v/v) glycerol.

 

 

 

 

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