Recombinant HIV-1 GAG Clade B

Recombinant HIV-1 GAG is a full length protein produced in E.coli. HIV Gag protein is encoded by the HIV gag gene, HXB2 nucleotides 790-2292. The 55-kDa Gag precursor is called assemblin to indicate its role in viral assembly.

SKU: REP0055 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 50 µg

Description

The Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell and contains four functional segments: Matrix (MA), Capsid (CA), Nucleocapsid (NC), and p6. The NC region is flanked by two “spacer” segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains.
Human immunodeficiency virus (HIV) and other primate lentiviruses assemble at the plasma membrane and are released by budding from the cell surface. This mode of assembly allows the viral capsid to acquire a host cell-derived lipid envelope which protects it from the environment. HIV-1 assembly is controlled primarily by the Gag protein. Gag encodes the capsid proteins (group specific antigens), which are the core structural proteins of HIV. HIV Gag protein is encoded by the HIV gag gene, HXB2 nucleotides 790-2292. The precursor is the p55 myristoylated protein, which is processed to p17 (MAtrix), p24 (CApsid), p7 (NucleoCapsid), and p6 proteins, by the viral protease (PR). Gag associates with the plasma membrane, where virus assembly takes place. The 55-kDa Gag precursor is called assemblin to indicate its role in viral assembly.

Additional information

Size 50 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category HIV
Subcategory Gag polyprotein

Technical information

Accession number GenBank AAD39400 Uniprot Q9WPY4
Size 50 µg
Expression system E.coli
Tag His- tagged
Purity >90%
Form Lyophilized with 1% glycerol.
Tested application SDS-PAGE, WB
Solubility The protein should be reconstituted in apirogenic sterile water or 1X PBS
Shipping/storage conditions Shipped at RT The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.
Usage For Laboratory Research Use Only

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