Recombinant HIV-1 GAG is a full length protein produced in E.coli. HIV Gag protein is encoded by the HIV gag gene, HXB2 nucleotides 790-2292. The 55-kDa Gag precursor is called assemblin to indicate its role in viral assembly.
Recombinant HIV-1 GAG Clade B
Description
The Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell and contains four functional segments: Matrix (MA), Capsid (CA), Nucleocapsid (NC), and p6. The NC region is flanked by two “spacer” segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains.
Human immunodeficiency virus (HIV) and other primate lentiviruses assemble at the plasma membrane and are released by budding from the cell surface. This mode of assembly allows the viral capsid to acquire a host cell-derived lipid envelope which protects it from the environment. HIV-1 assembly is controlled primarily by the Gag protein. Gag encodes the capsid proteins (group specific antigens), which are the core structural proteins of HIV. HIV Gag protein is encoded by the HIV gag gene, HXB2 nucleotides 790-2292. The precursor is the p55 myristoylated protein, which is processed to p17 (MAtrix), p24 (CApsid), p7 (NucleoCapsid), and p6 proteins, by the viral protease (PR). Gag associates with the plasma membrane, where virus assembly takes place. The 55-kDa Gag precursor is called assemblin to indicate its role in viral assembly.
Additional information
| Size | 50 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Recombinant protein |
| Category | HIV |
| Subcategory | Gag polyprotein |
Technical information
| Accession number | GenBank AAD39400 Uniprot Q9WPY4 |
|---|---|
| Size | 50 µg |
| Expression system | E.coli |
| Tag | His- tagged |
| Purity | >90% |
| Form | Lyophilized with 1% glycerol. |
| Tested application | SDS-PAGE, WB |
| Solubility | The protein should be reconstituted in apirogenic sterile water or 1X PBS |
| Shipping/storage conditions | Shipped at RT The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles. |
| Usage | For Laboratory Research Use Only |
