HIV-1 Gag p24 antigen is a capsid protein that constitutes the core of HIV-I. p24 protein is detectable starting from the early stage of AIDS virus infection, for this reason it is an interesting marker for disease progression in infected patients. Monoclonal anti p24 antibody clone 4 is designed for the detection of p24 core antigen in human serum or plasma from infected individuals as well as in cell culture supernatant.
Mouse Anti HIV-I p24 (Clone 4)
Testing of blood donor samples for HIV-1 antigens (p24) was implemented in the United States in 1996 in addition to HIV antibody testing. This decision was based on studies showing that HIV-1 antigen appears in blood early in the course of infection, approximately 1 week before antibody is detectable. Transmission of HIV has been reported from transfusion of seronegative blood that was later shown to contain p24 antigen; the donors subsequently showed seroconversion. Mathematical models, constructed with findings from geographic areas with very high incidence of new HIV infections, suggested that routine antigen testing would detect 1 antigen-positive/antibody-negative donation in every 1.6 million tested. In reality, this projection was not achieved. Only seven donations from infected window-phase (p24 antigen-positive/anti-HIV-negative) volunteer donors were intercepted on the basis of p24 antigen testing alone in the first 5 years of screening, for an observed yield of 1 in 10 million donations. A possible explanation for the underperformance of HIV-1 antigen testing in detecting HIV-1 antibody-negative window period infections is that prospective blood donors in the early phase of HIV-1 infection who have HIV-1 antigen only may be experiencing symptoms from acute retroviral infection that deter them from blood donation or result in their rejection during the predonation history and physical examination.
|Application||Clinical diagnostics| Life Sciences|
|Product type||Reagents for research|
|Subcategory||Mouse monoclonal IgG1 with a k light chain|
|Antibody type||Primary antibody|
|Immunogen||Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 gag recombinant protein (clade B, HXB-3 isolate)|
|Purity||Purified from supernatants of hybridoma cell cultures by affinity chromatography|
|Form||Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.|
|Shipping/storage conditions||Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles.|
|Usage||For Laboratory Research Use Only|
|Specificity||p24 from HIV-1 virus in human specimens|
|React with||HIV-1 p24 protein|
|Recommended dilutions||Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values.|
|IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation||PUBMED ID: 26185770|