HIV-1 DNA Test PRO

The HIV-1 DNA Test PRO is a real-time PCR assay based on dual-labelled probes that allows for the quantification of total HIV-1 DNA in blood and PBMC samples from HIV-1 positive patients. The provided mix alllows the simultaneous quantitation of the endogeneous hTERT target. The expression of the results can be therefore normalized for the cell content and expressed as HIV-1 copy number per cells.

SKU: MBK0087-32T Categories: , ,
Application: Clinical Diagnostics
Product type: Diagnostic kit
Reagent type: PCR
Size: 32 tests

Description

HIV-1 DNA is an innovative and widely used marker of HIV persistence in infected cells. It is known that HIV-1 DNA levels vary among patients according to the stages of HIV disease and the effectiveness of anti-HIV therapy. Total HIV-1 DNA includes an overall quantification of all viral forms of HIV DNA in infected cells, each playing a different role in HIV replication and pathophysiology, monitoring the number of infected cells in a latent or productive state. The kit provides PCR mix that offers robust and consistent performances even in presence of PCR inhibitors and a stringent automatic hot-start, allowing reaction assembly and temporary storage at room temperature prior to PCR amplification. The ready-to-use duplex real-time PCR mix is specific for the amplification of both HIV-1 DNA and the endogenous reference human Telomerase Reverse Transcriptase gene (hTERT), required for relative quantification of HIV-1 copy number. Moreover the kit includes a ready-to-use standard curve with 5 levels of HIV-1 copies and cell content. QUANTITATIVE TEST – Monitor the HIV-1 reservoirs and study of the relationship between the amount of HIV DNA and residual viremia in patients treated with ART and during changes of the therapy; monitor of the infection in aviremic patients, for whom the amount of proviral DNA is the only quantifiable parameter; QUALITATIVE TEST – Diagnosis and follow-up of the first 18-24 months of life of children born from HIV-positive mothers.

Additional information

Size 32 tests
Application Clinical diagnostics
Product type Diagnostic kit
Reagent type PCR
Category HIV

Technical information

Size 32 tests
Technology Real-time PCR
Specimen Human blood and PBMC samples
Testing time 3 hours
Target gene LTR (for HIV) hTERT (endogenous gene)

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References

Development of C-TILDA: A modified TILDA method for reservoir quantification in long term treated patients infected with subtype C HIV-1 PUBMED ID: 31756409

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HIV-1 DNA Test PRO
The HIV-1 DNA Test PRO is a real-time PCR assay based on dual-labelled probes that allows for the quantification of total HIV-1 DNA in blood and PBMC samples from HIV-1 positive patients. The provided mix alllows the simultaneous quantitation of the endogeneous hTERT target. The expression of the results can be therefore normalized for the cell content and expressed as HIV-1 copy number per cells.
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The HIV-1 DNA Test is a real-time PCR assay that allows the quantification of total HIV-1 DNA in blood and PBMC samples from HIV-1 positive patients.
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