Recombinant TAT CRF02_AG

Recombinant (HIV-1) CRF02_AG Tat produced in E.coli. The HIV-1 CFR02_AG Tat protein is a molecule of 101 aminoacids encoded by two exons and having a molecular mass of 20.1kDa in SDS-PAGE.

SKU: REP0059 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 100 µg

Description

HIV-1 circulating recombinant forms (CRFs) represent viral recombinant lineages that play a significant role in the global epidemic. Two of them dominate the epidemic in Burkina Faso: CRF06_cpx (first described in this country) and CRF02_AG. We reconstructed the phylodynamics of both recombinant viruses in Burkina Faso and throughout West Africa..(Infect Genet Evol. 2016 Yebra G1, Kalish ML2, Leigh Brown AJ3.)
Multiple HIV-1 subtypes and circulating recombinant forms (CRFs) are known to cocirculate in Africa. In West Africa, the high prevalence of CRF02_AG and cocirculation of subtype A, CRF01_AE, CRF06_cpx, and other complex intersubtype recombinants has been well documented. Cocirculation of CRF02_AG strain with other genetic subtypes may give rise to novel and complex HIV-1 recombinants.

Additional information

Size 100 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category HIV
Subcategory TAT Protein (Transactivating regulatory protein)

Technical information

Accession number GenBank AY371128 (truncated form)
Organism HIV-1 isolate 02CM.1970LE from Cameroon gag protein (gag) and pol protein (pol) genes, partial cds; vif protein (vif), vpr protein (vpr), tat protein (tat), rev protein (rev), vpu protein (vpu), and envelope glycoprotein (env) genes, complete cds; and nef protein (nef) gene, partial cd.
Size 100 µg
Potency Not biologically Active protein
Expression system E.coli
Tag No tags
Purity >90%
Form Lyophilized with 0.1% glycerol.
Tested application WB, SDS-PAGE, Endotoxins, Up-take, Rescue assay
Solubility The protein should be reconstituted in apirogenic sterile water or 1X PBS
Shipping/storage conditions Shipped at RT. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.
Usage For Laboratory Research Use Only

Download

References

Differential mechanisms of inflammation and endothelial dysfunction by HIV-1 subtype-B and recombinant CRF02_AG Tat proteins on human brain microvascular endothelial cells: implications for viral neuropathogenesis. PUBMED ID: 28127697
Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses Biju Bhargavan & Georgette D. Kanmogne Scientific Reports volume 9, Article number: 10689 (2019) PUBMED ID: 31337802

Related products

Recombinant TAT Clade B BIOTIN
HIV-1 TAT Recombinant Tat Clade B Biotein conjugated, is produced in E.coli and conjugated with NHS Biotin.
REP0004
Recombinant TAT Clade B
HIV-1 TAT Recombinant- produced in E.coli is a single, non-glycosylated, polypeptidic chain containing 86 amino acids encoded by two exons and having a molecular mass of 14kDa.
REP0002a
Recombinant TAT Cys22
HIV-1 regulatory Tat protein plays an essential role in viral replication and infectivity. In addition, during acute infection, Tat is released extracellularly by infected cells and is taken up by neighbouring cells where it transactivates viral replication and increases virus infectivity. The Tat cys22 protein of HIV-1 is a molecule of 86 aminoacids mutated in the trans activation domain.
REP0033
Recombinant TAT Clade C
Recombinant (HIV-1) Tat Clade C produced in E.coli. The HIV-1 Tat protein Clade C is a molecule of 101 aminoacids encoded by two exons.
REP0030
Recombinant TAT Clade A
Recombinant (HIV-1) Tat clade A produced in E. coli. Productive HIV-1 replication is absolutely dependent upon the viral tat protein. In Uganda Clades A and D are responsible for approximately 95% of HIV-1 infections. The HIV-1 tat protein Clade A is a molecule of 86 aminoacids encoded by two exons and having a molecular mass of 20.1 kDa in SDS-PAGE
REP0043
Recombinant TAT Clade B C27A-C31A Mutant
Recombinant HIV-1 TAT Clade B C31A-C34A is produced in E.coli and is mutate in the cystein-rich region. It Is a single, non-glycosylated, polypeptidic chain containing 86 amino acids encoded by two exons.
REP0061
Recombinant TAT CRF02_AG
Recombinant (HIV-1) CRF02_AG Tat produced in E.coli. The HIV-1 CFR02_AG Tat protein is a molecule of 101 aminoacids encoded by two exons and having a molecular mass of 20.1kDa in SDS-PAGE.
REP0059
Rabbit Anti HIV-I Tat
The HIV-1 regulatory protein Tat is considered an attractive target for the development of a multicomponent vaccine against HIV-1 infection. The rabbit polyclonal Anti HIV-1 Tat antibody is a useful tool for detecting Tat transactivating regulatory protein
ANT0001
Rabbit Anti HIV-1 Tat Biotin conjugated
The rabbit polyclonal Anti HIV-1 Tat antibody Biotin conjugated is a useful tool for detecting Tat transactivating regulatory protein. The conjugation garantees an improvement on the specific signal and the best signal on semidirect immunological methods
ANT0003
Rabbit Anti HIV-I Tat FITC conjugated
The rabbit polyclonal Anti HIV-1 Tat antibody FITC conjugated is a useful tool for detecting Tat transactivating regulatory protein. The florophore conjugation garantees an improvement on the specific signal and the best signal on fluorescence methodologies
ANT0005
Rabbit Anti HIV-I Tat affinity purified
The rabbit polyclonal Anti HIV-1 Tat antibody affinity purified is a useful tool for detecting Tat transactivating regulatory protein. The affinity purification protocol garantees an improvement on the specific signal
ANT0041

Information request

    Go to Top