The specific antibodies against different subunits of spike (S) protein of SARS-CoV-2 are excellent research tools for research against coronavirus. Anti SARS CoV-2 Spike2 monoclonal antibody detects conformational SARS CoV-2 Spike2. The antibody does not cross react with SARS CoV-2 Spike1 and SARS CoV-2 Spike1-RBD domain.
SARS-CoV-2 Spike S2 monoclonal antibody (S223)
Description
Coronavirus disease 2019 is a newly emerging infectious disease currently spreading across the world. It is caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The spike (S) protein of SARS-CoV-2, which plays a key role in the receptor recognition and cell membrane fusion process, is composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain that recognizes and binds to the host receptor angiotensin-converting enzyme 2, while the S2 subunit mediates viral cell membrane fusion by forming a six-helical bundle via the two-heptad repeat domain. The specific antibodies against different subunits of spike (S) protein of SARS-CoV-2 are excellent research tools for research against coronavirus
Additional information
| Size | 100 μg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Antibody |
| Category | Covid-19 |
| Subcategory | Monoclonal antibody |
| Antibody type | Primary antibody |
| Immunogen | Conformational SARS-CoV-2 Spike2 domain |
Technical information
| Size | 100g |
|---|---|
| Purity | Mouse monoclonal immunoglobulins IgG1 subclass were purified by protein L affinity chromatography and stabilized with 0.05% of glycerol. |
| Form | Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH. |
| Shipping/storage conditions | Shipped at -20°C When stored at -20°C, the antibody is stable for 12 months. |
| Usage | For Laboratory Research Use Only |
| Specificity | Anti SARS CoV-2 Spike2 monoclonal antibody detects conformational SARS CoV-2 Spike2. The antibody does not cross react with SARS CoV-2 Spike1 and SARS CoV-2 Spike1-RBD domain |
| React with | Conformational SARS-CoV-2 Spike2 domain |
| Additional notes | When using any primary antibody or fluorescence-labelled secondary antibody for the first time, titrate out the antibody to determine which dilution allows the strongest specific signal with the lowest background for your sample. Avoid repeated freezing and thawing cycles. It is recommended aliquoting the product upon arrival. |
| Recommended dilutions | Recommended starting dilutions can vary lot-to-lot. |
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References
| Clinical and Analytical Performance of an Automated Serological Test That Identifies S1/S2-Neutralizing IgG in COVID-19 Patients Semiquantitatively. | doi: 10.1128/JCM.01224-20 |
| Structures and distributions of SARS-CoV-2 spike proteins on intact virions. | doi: 10.1038/s41586-020-2665-2 |
| SARS-CoV-2-specific virulence factors in COVID-19. | doi: 10.1002/jmv.26615 |
