The rabbit polyclonal Anti HIV-1 Tat antibody affinity purified is a useful tool for detecting Tat transactivating regulatory protein. The affinity purification protocol garantees an improvement on the specific signal
Rabbit Anti HIV-I Tat affinity purified
Description
The HIV-1 regulatory protein Tat is considered an attractive target for the development of a multicomponent vaccine against HIV-1 infection. This protein is considered an attractive target for the development of a multicomponent vaccine against HIV-1 infection. The protein is well conserved among isolates and thus may be less susceptible to mutation leading to the production of escape virus variants. Tat is produced early after infection and it’s essential for virus replication and infectivity. Tat protein is also immunogenic and antibody against Tat have been correlated with delayed disease progression and may exert protective effects inhibiting HIV-1 replication. Moreover, Tat is efficiently taken up by monocyte-derived dentritic cells, promoting their maturation. Finally, murine vaccination wwith a biologically active Tat protein has been shown to be safe and immunogenic.
Additional information
| Size | 200 µL |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Antibody |
| Category | HIV |
| Subcategory | Polyclonal Anti Tat antibody purified against antigen |
| Antibody type | Primary antibody |
| Immunogen | Recombinant purified Tat protein (HIV-1 Clade B) expressed in E.coli |
Technical information
| Accession number | UniProt P69697.1 |
|---|---|
| Size | 200 µL |
| Purity | Purified by affinity Chromatography on Tat antigen |
| Form | Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH. |
| Shipping/storage conditions | Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles. |
| Usage | For Laboratory Research Use Only |
| Source | Rabbit |
| Specificity | Tat protein clade A and B, GST-Tat fusion protein |
| React with | Tat from HIV-1 |
| Recommended dilutions | Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values. |
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References
| DNA Vectors Generating Engineered Exosomes Potential CTL Vaccine Candidates Against AIDS, Hepatitis B, and Tumors | PUBMED ID: 30167966 |
| Trans-dissemination of exosomes from HIV-1-infected cells fosters both HIV-1 trans-infection in resting CD4+ T lymphocytes and reactivation of the HIV-1 reservoir | PUBMED ID: 28474225 |
| The EGF epidermal growth factor counteracts Tat modulation of human endogenous retroviruses of the W family in astrocytes | PUBMED ID: 28474333 |
| Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels | PUBMED ID: 27698804 |
