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Recombinant TAT Clade B C31A-C34A Mutant

Recombinant HIV-1 TAT Clade B C31A-C34A is produced in E.coli and is mutate in the cystein-rich region. It Is a single, non-glycosylated, polypeptidic chain containing 86 amino acids encoded by two exons.

SKU: REP0062 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 50 µg

Description

The redox state of cysteine-rich region of tat protein is known to play a crucial role in tat biological activity. The cysteine-rich region, encompassing seven cysteine residues, is highly conserved among the different HIV-1 strains and isolates. Its redox state is known to be important for protein function and activity. In particular, variation of tat cysteine motif known as “cysteine 30-cysteine 31” (C30C31) is associated with clade-specific tat neurotoxicity.
HIV-1 regulatory tat protein plays an essential role in viral replication and infectivity. In addition, during acute infection tat is released in the extracellular environment by infected cells and is taken up by neighbouring cells where it transactivates viral replication and increases virus infectivity. The HIV-1 tat protein is a molecule of 86 aminoacids encoded by two exons.

Additional information

Size 50 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category HIV
Subcategory TAT Protein (Transactivating regulatory protein)

Technical information

Size 50 µg
Potency Not biologically Active protein
Expression system E.coli
Tag No tags
Purity >90%
Form Lyophilized with 0.1% glycerol.
Tested application WB, SDS-PAGE, Endotoxins, Up-take, Rescue assay
Solubility The protein should be reconstituted in apirogenic sterile water or 1X PBS
Shipping/storage conditions Shipped in dry ice. The lyophilized protein is stable for 24 months if stored at -80°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.
Usage For Laboratory Research Use Only

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