Recombinant NEF Clade B is a full length protein produced in E.coli. The HIV -1 Nef Clade B is a single non-glycosylated polypeptidic chain with a molecular mass of 27 kDa.
Recombinant NEF Clade B
HIV-1 Nef is a 27 kDa protein abundantly produced shortly after virus infection. Nef is an essential factor for efficient viral replication and pathogenesis, it also facilitates virus replication and enhances virions infectivity. Nef exerts pleiotropic effects: it down-modulates surface MHC-I molecules, decreases cell surface CD4 antigen by interacting with the Src family kinase LCK, protects the infected cell from apoptosis in order to keep it alive until the next virus generation is ready to strike. Nef protein bypasses host T-cell signaling by inducing a trascriptional program nearly identical to that of anti-CD3 cell activation.
|Application||Clinical diagnostics| Life Sciences|
|Product type||Reagents for research|
|Reagent type||Recombinant protein|
|Accession number||GenBank CAA41585|
|Organism||Human immunodeficiency virus 1|
|Form||Lyophilized with 1% glycerol.|
|Tested application||SDS-PAGE, HPLC, Endotoxin|
|Solubility||The protein should be reconstituted in apirogenic sterile water or 1X PBS|
|Shipping/storage conditions||Shipped at RT. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.|
|Usage||For Laboratory Research Use Only|