Recombinant NEF Clade B

Recombinant NEF Clade B is a full length protein produced in E.coli. The HIV -1 Nef Clade B is a single non-glycosylated polypeptidic chain with a molecular mass of 27 kDa.

SKU: REP0035 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 50 µg


HIV-1 Nef is a 27 kDa protein abundantly produced shortly after virus infection. Nef is an essential factor for efficient viral replication and pathogenesis, it also facilitates virus replication and enhances virions infectivity. Nef exerts pleiotropic effects: it down-modulates surface MHC-I molecules, decreases cell surface CD4 antigen by interacting with the Src family kinase LCK, protects the infected cell from apoptosis in order to keep it alive until the next virus generation is ready to strike. Nef protein bypasses host T-cell signaling by inducing a trascriptional program nearly identical to that of anti-CD3 cell activation.

Additional information

Size 50 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category HIV
Subcategory Nef protein

Technical information

Accession number GenBank CAA41585
Organism Human immunodeficiency virus 1
Size 50 µg
Expression system E.coli
Tag No tags
Purity >90%
Form Lyophilized with 1% glycerol.
Tested application SDS-PAGE, HPLC, Endotoxin
Solubility The protein should be reconstituted in apirogenic sterile water or 1X PBS
Shipping/storage conditions Shipped at RT. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.
Usage For Laboratory Research Use Only



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Recombinant NEF BIOTIN
Recombinant HIV-1 Nef Clade B is a full length protein produced in E.coli biotin conjugated. The HIV -1 Nef Clade B is a single non-glycosylated polypeptide chain with a molecular mass of 27kDa.

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