Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration. The process of integration can be divided into two sequential reactions. The first one, named 3′-processing, corresponds to a specific endonucleolytic reaction which prepares the viral DNA extremities to be competent for the subsequent covalent insertion, named strand transfer, into the host cell genome by a trans-esterification reaction. The monoclonal antibody is an aid in the reasearch against HIV-1 and in the diagnostic test development.
Mouse Anti HIV-I Integrase (Clone 2)
Integration of viral DNA into a chromosome of the host cell is an essential step in the retroviral life cycle. This process is catalyzed by the viral enzyme integrase (IN) through 3 steps: first step, two nucleotides are removed from the 39 ends of the viral DNA (39-end processing); second step, the recessed 39 ends of the viral DNA are then joined to 59 staggered sites in the target DNA in a concerted cleavage and ligation reaction (DNA joining); last step, integration is completed by repair of the short gaps flanking the viral DNA intermediate and subsequent joining of the 59 ends of viral DNA to the target DNA. HIV integrase contains 3 domains, an N-terminal HH-CC zinc-finger domain which is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both central catalytic domain and C-terminal domain have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimer or tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.
|Application||Clinical diagnostics| Life Sciences|
|Product type||Reagents for research|
|Subcategory||Mouse monoclonal IgG1 with a k light chain|
|Antibody type||Primary antibody|
|Immunogen||Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate)|
|Purity||Purified from supernatants of hybridoma cell cultures by affinity chromatography|
|Form||Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.|
|Shipping/storage conditions||Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles.|
|Usage||For Laboratory Research Use Only|
|Specificity||Integrase from HIV-1 virus in human specimens.|
|React with||HIV-1 Integrase protein|
|Recommended dilutions||Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values.|
|Novel 3'-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors||PUBMED ID: 26064960|