Recombinant HIV-1 Integrase is a His tagged protein produced in E.coli. The HIV -1 Integrase is a molecule of 288 amino acids with a molecular mass of 32kDa.
Recombinant Integrase
Description
Integrase catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. The enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The full length HIV-1 integrase (288 amino acids) has three domains: the catalytic core, the C-terminal, and the N-terminal domains. Although all three domains are required for integration, it is thought that the catalytic core domain contains the active site responsible for catalysis of all the reactions of integration/disintegration.
Additional information
| Size | 20 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Recombinant protein |
| Category | HIV |
| Subcategory | Integrase |
Technical information
| Accession number | GenBank ACM44577.2 |
|---|---|
| Organism | Integrase partial [Human immunodeficiency virus 1] |
| Size | 20 µg |
| Expression system | E.coli |
| Tag | His tagged |
| Purity | >95% |
| Form | Liquid in 20mM phosphate buffer pH 7.5, 1M NaCl, 10µM Zinc acetate, 1mM DTT, 0.1mM EDTA; 10%(v/v) glycerol. |
| Tested application | SDS-PAGE, WB |
| Shipping/storage conditions | Shipped in dry ice. The protein is stable for 12 months if stored at -20°C. Avoid freeze-thaw cycles. |
| Usage | For Laboratory Research Use Only |
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References
| Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors | PUBMED ID: 26064960 |
