Recombinant Integrase

Recombinant HIV-1 Integrase is a His tagged protein produced in E.coli. The HIV -1 Integrase is a molecule of 288 amino acids with a molecular mass of 32kDa.

SKU: REP0063 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 20 µg

Description

Integrase catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. The enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The full length HIV-1 integrase (288 amino acids) has three domains: the catalytic core, the C-terminal, and the N-terminal domains. Although all three domains are required for integration, it is thought that the catalytic core domain contains the active site responsible for catalysis of all the reactions of integration/disintegration.

Additional information

Size 20 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category HIV
Subcategory Integrase

Technical information

Accession number GenBank ACM44577.2
Organism Integrase partial [Human immunodeficiency virus 1]
Size 20 µg
Expression system E.coli
Tag His tagged
Purity >95%
Form Liquid in 20mM phosphate buffer pH 7.5, 1M NaCl, 10µM Zinc acetate, 1mM DTT, 0.1mM EDTA; 10%(v/v) glycerol.
Tested application SDS-PAGE, WB
Shipping/storage conditions Shipped in dry ice. The protein is stable for 12 months if stored at -20°C. Avoid freeze-thaw cycles.
Usage For Laboratory Research Use Only

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References

Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors PUBMED ID: 26064960

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