Recombinant (HIV-1) CRF02_AG Tat produced in E.coli. The HIV-1 CFR02_AG Tat protein is a molecule of 101 aminoacids encoded by two exons and having a molecular mass of 20.1kDa in SDS-PAGE.
Recombinant TAT CRF02_AG
Description
HIV-1 circulating recombinant forms (CRFs) represent viral recombinant lineages that play a significant role in the global epidemic. Two of them dominate the epidemic in Burkina Faso: CRF06_cpx (first described in this country) and CRF02_AG. We reconstructed the phylodynamics of both recombinant viruses in Burkina Faso and throughout West Africa.(Infect Genet Evol. 2016 Yebra G1, Kalish ML2, Leigh Brown AJ3.)
Multiple HIV-1 subtypes and circulating recombinant forms (CRFs) are known to cocirculate in Africa. In West Africa, the high prevalence of CRF02_AG and cocirculation of subtype A, CRF01_AE, CRF06_cpx, and other complex intersubtype recombinants has been well documented. Cocirculation of CRF02_AG strain with other genetic subtypes may give rise to novel and complex HIV-1 recombinants.
Additional information
| Size | 50 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Recombinant protein |
| Category | HIV |
| Subcategory | TAT Protein (Transactivating regulatory protein) |
Technical information
| Accession number | GenBank AY371128 (truncated form) |
|---|---|
| Organism | HIV-1 isolate 02CM.1970LE from Cameroon gag protein (gag) and pol protein (pol) genes, partial cds; vif protein (vif), vpr protein (vpr), tat protein (tat), rev protein (rev), vpu protein (vpu), and envelope glycoprotein (env) genes, complete cds; and nef protein (nef) gene, partial cd. |
| Size | 50 µg |
| Potency | Not biologically Active protein |
| Expression system | E.coli |
| Tag | No tags |
| Purity | >90% |
| Form | Lyophilized with 0.1% glycerol. |
| Tested application | WB, SDS-PAGE, Endotoxins, Up-take, Rescue assay |
| Solubility | The protein should be reconstituted in apirogenic sterile water or 1X PBS |
| Shipping/storage conditions | Shipped at RT. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles. |
| Usage | For Laboratory Research Use Only |
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References
| Differential mechanisms of inflammation and endothelial dysfunction by HIV-1 subtype-B and recombinant CRF02_AG Tat proteins on human brain microvascular endothelial cells: implications for viral neuropathogenesis. | PUBMED ID: 28127697 |
| Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses | PUBMED ID: 31337802 |
