The HIV-1 Rev protein mediates the nuclear export of viral mRNA, and is thereby essential for the production of late viral proteins in the replication cycle. Rev forms a large organized multimeric protein-protein complex for proper functioning. Monoclonal anti HIV-1 REV antibody Clone 6 is designed for the detection of REV in human serum or plasma from infected individuals as well as in cell culture supernatant.
Mouse Anti HIV-I Rev (Clone 6)
Three major regions of the Rev protein are required for it to help HIV replication: an RNA binding region, an oligomerization region, and a Nuclear Export Signal. The RNA binding region interacts with a part of the viral RNA called the Rev-Responsive Element (RRE). A small (∼30 nucleotides) binding site within this element has a high affinity for Rev, forming a larger complex. The RNA binding region of Rev forms an alpha helix that docks into a complementary groove in the high affinity binding site on RRE . Adding more Rev proteins onto the RRE results in the formation of a series of larger complexes, but where these additional Rev molecules bind is not known yet. The one exception is a weak secondary Rev binding site that has been identified adjacent to the high affinity site.
|Application||Clinical diagnostics| Life Sciences|
|Product type||Reagents for research|
|Subcategory||Mouse monoclonal IgG1 with a k light chain|
|Antibody type||Primary antibody|
|Immunogen||HIV-1 Rev recombinant protein His-6 Tag (Clade B, HXB-3 isolate)|
|Purity||Purified from supernatants of hybridoma cell cultures by affinity chromatography|
|Form||Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.|
|Shipping/storage conditions||Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles.|
|Usage||For Laboratory Research Use Only|
|Specificity||REV protein from HIV-1 virus in human specimens|
|React with||REV HIV-1 viral protein|
|Recommended dilutions||Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values.|