The HIV-1 Rev protein mediates the nuclear export of viral mRNA, and is thereby essential for the production of late viral proteins in the replication cycle. Rev forms a large organized multimeric protein-protein complex for proper functioning. Monoclonal anti HIV-1 REV antibody Clone 6 is designed for the detection of REV in human serum or plasma from infected individuals as well as in cell culture supernatant.
Mouse Anti HIV-I Rev (Clone 6)
Description
Three major regions of the Rev protein are required for it to help HIV replication: an RNA binding region, an oligomerization region, and a Nuclear Export Signal. The RNA binding region interacts with a part of the viral RNA called the Rev-Responsive Element (RRE). A small (∼30 nucleotides) binding site within this element has a high affinity for Rev, forming a larger complex. The RNA binding region of Rev forms an alpha helix that docks into a complementary groove in the high affinity binding site on RRE . Adding more Rev proteins onto the RRE results in the formation of a series of larger complexes, but where these additional Rev molecules bind is not known yet. The one exception is a weak secondary Rev binding site that has been identified adjacent to the high affinity site.
Additional information
| Size | 50 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Antibody |
| Category | HIV |
| Subcategory | Mouse monoclonal IgG1 with a k light chain |
| Antibody type | Primary antibody |
| Immunogen | HIV-1 Rev recombinant protein His-6 Tag (Clade B, HXB-3 isolate) |
Technical information
| Size | 50 µg |
|---|---|
| Purity | Purified from supernatants of hybridoma cell cultures by affinity chromatography |
| Form | Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH. |
| Shipping/storage conditions | Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles. |
| Usage | For Laboratory Research Use Only |
| Source | Mouse |
| Specificity | REV protein from HIV-1 virus in human specimens |
| React with | REV HIV-1 viral protein |
| Recommended dilutions | Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values. |
