Recombinant Prelamin A is a protein produced in E. coli. The Prelamin A is a 74kDa precursor of the nuclear lamin A protein, with N-terminal His tag and DDDDK tag.
Recombinant Human Prelamin A
Description
Recombinant prelamin A is a 74 kDa precursor of the nuclear lamin A protein, that is a structural component of the nuclear lamina encoded by lamin A/C gene (LMNA). Due to the presence of a CAAX box sequence at carboxyl terminus, prelamin A in vivo undergoes to serial post-translational modifications, resulting in the farnesylation of the cysteine thiol, removal of the AAX tripeptide, carboxyl-methylation of the cysteinyl carboxyl group and proteolysis of 18 C-terminal amino acids residues that lead to mature lamin A. Different mutations in the LMNA gene have been shown to be associated with different diseases collectively called laminophaties, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Additional information
| Size | 100 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Recombinant protein |
| Category | Laminopathies |
Technical information
| Accession number | GenBank NP_733821 |
|---|---|
| Organism | Human |
| Size | 100 µg |
| Expression system | E.coli |
| Tag | His tagged |
| Purity | >90% |
| Form | 20mM phosphate buffer pH 7.0, 0.5M NaCl, 1mM DTT, 1.5mM EDTA, 20%(v/v) glycerol. |
| Tested application | SDS-PAGE, WB |
| Shipping/storage conditions | Shipped in dry ice . Upon delivery aliquot and store at -80°C. Avoid freeze-thaw cycles. |
| Usage | For Laboratory Research Use Only |
