Rabbit Anti Human Prelamin A

A-Type lamins are nuclear proteins required for the structural and functional integrity of the nucleus. In mammals, type A proteins are represented by lamins A and C, which are different transcripts arising from the same gene LMNA. This polyclonal antibody is able to detect full-lenght human prelamin A.

SKU: ANT0045 Categories: , , ,
Product type: Reagents for research
Reagent type: Antibody
Size: 200 µL

Description

Several laminopathies are characterized by anomalous accumulation of prelamin A. Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. These proteins have an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. It also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Mutations in lamin A produce a range of diseases that have collectively been referred to as `laminopathies’ . Two progeroid (premature aging) syndromes, Hutchinson-Gilford progeria (HGPS) and restrictive dermopathy (RD), are laminopathies that arise through defects in maturation of the lamin A precursor, prelamin A. Mandibuloacral dysplasia (MAD), which can be considered a milder form of RD, also results in accumulation of the lamin A precursor. By contrast, the majority of laminopathies are due to point mutations in the A/C lamins; these exhibit a multitude of phenotypes depending on the site of the mutation .

Additional information

Size 200 µL
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Antibody
Category Laminopathies
Subcategory Polyclonal anti human Prelamin A
Antibody type Primary antibody
Immunogen Prelamin A A C-terminus peptide

Technical information

Size 200 µL
Purity Purified by affinity Chromatography
Form Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.
Shipping/storage conditions Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles.
Usage For Laboratory Research Use Only
Source Rabbit
Specificity Antibody binds Prelamin A in cells lines and in patient-derived specimens
React with Full-lenght Human Prelamin A
Recommended dilutions Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values.

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References

Nuclear Nox4 interaction with prelamin A is associated with nuclear redox control of stem cell aging PUBMED ID: 30362963
Samp1 Mislocalization in Emery-Dreifuss Muscular Dystrophy PUBMED ID: 30326651
The relevance of prelamin A and RAD51 as molecular biomarkers in cervical cancer PUBMED ID: 29212225
Statins and Histone Deacetylase Inhibitors Affect Lamin A/C - Histone Deacetylase 2 Interaction in Human Cells PUBMED ID: 30766871
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Recombinant Human Prelamin A
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Rabbit Anti Human Cleaved Farnesylated Prelamin A
The antibody is able to detect cleaved farnesylated prelamin A, while it does not recognize either full-length prelamin A or mature lamin A. This antibody can be used for the discrimination of prelamin A forms and to check the efficacy of potential therapeutic compounds acting on prelamin A processing.
ANT0046
Rabbit Anti Human Lamin A
A-Type lamins are nuclear proteins required for the structural and functional integrity of the nucleus. In mammals, type A proteins are represented by lamins A and C, which are different transcripts arising from the same gene LMNA. This polyclonal antibody is able to detect full-lenght prelamin A and human Lamin A.
ANT0072
Rabbit Anti Human Prelamin A Biotin conjugated
A-Type lamins are nuclear proteins required for the structural and functional integrity of the nucleus. In mammals, type A proteins are represented by lamins A and C, which are different transcripts arising from the same gene LMNA. This polyclonal antibody is able to detect full-lenght human prelamin A. The antibody Biotin conjugation garantees an improvement on the specific signal and the best signal on semidirect immunological methods
ANT0078
Rabbit Anti Human Lamin A Biotin conjugated
A-Type lamins are nuclear proteins required for the structural and functional integrity of the nucleus. In mammals, type A proteins are represented by lamins A and C, which are different transcripts arising from the same gene LMNA. This polyclonal antibody is able to detect full-lenght human prelamin A and Lamin A.
ANT0079

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