Recombinant Human Prelamin A

Recombinant Prelamin A is a protein produced in E. coli. The Prelamin A is a 74kDa precursor of the nuclear lamin A protein, with N-terminal His tag and DDDDK tag.

SKU: REP0039 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 50 µg

Description

Recombinant prelamin A is a 74 kDa precursor of the nuclear lamin A protein, that is a structural component of the nuclear lamina encoded by lamin A/C gene (LMNA). Due to the presence of a CAAX box sequence at carboxyl terminus, prelamin A in vivo undergoes to serial post-translational modifications, resulting in the farnesylation of the cysteine thiol, removal of the AAX tripeptide, carboxyl-methylation of the cysteinyl carboxyl group and proteolysis of 18 C-terminal amino acids residues that lead to mature lamin A. Different mutations in the LMNA gene have been shown to be associated with different diseases collectively called laminophaties, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Additional information

Size 50 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category Laminopathies

Technical information

Accession number GenBank NP_733821
Organism Human
Size 50 µg
Expression system E.coli
Tag His tagged
Purity >90%
Form 20mM phosphate buffer pH 7.0, 0.5M NaCl, 1mM DTT, 1.5mM EDTA, 20%(v/v) glycerol.
Tested application SDS-PAGE,WB
Shipping/storage conditions Shipped in dry ice . Upon delivery aliquot and store at -80°C. Avoid freeze-thaw cycles.
Usage For Laboratory Research Use Only

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References

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