The recombinant Ag85B is a 325amino acids protein with a molecular weight of about 30 kDa and is produced in E.coli.
Recombinant Mycobacterium tuberculosis Ag85B
Description
The 30kD-secretory protein antigen 85B (Ag85B) is the most abundant protein exposed by Mycobacterium tuberculosis acting as a potent immunoprotective antigen. Ag85B induces a strong T-cell proliferation and the IFN- secretion in most healthy individuals exposed to M. tuberculosis, in BCG-vaccinated mice and humans. It has been shown that antibodies against Ag85B are more prevalent in active tuberculosis patients with decreased cellular immune response.
Additional information
| Size | 50 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Recombinant protein |
| Category | Mycobacterium tubercolosis |
| Subcategory | Ag85B |
Technical information
| Accession number | UniProtKB/Swiss-Prot: P9WQP0.1 |
|---|---|
| Organism | Mycobacterium tuberculosis |
| Size | 50 µg |
| Expression system | E.coli |
| Tag | HIS tagged |
| Purity | >90% |
| Form | Lyophilized. The protein should be reconstituted in apirogenic sterile water or 1X PBS. |
| Tested application | SDS-PAGE, Endotoxin |
| Shipping/storage conditions | Shipped at +RT°C. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. |
| Usage | For Laboratory Research Use Only |
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References
| Recombinant bacille Calmette–Guerin coexpressing Ag85b, CFP10, and interleukin-12 elicits effective protection against Mycobacterium tuberculosis |
