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Recombinant Mycobacterium tuberculosis Ag85B

The recombinant Ag85B is a 325amino acids protein with a molecular weight of about 30 kDa and is produced in E.coli.

SKU: REP0025 Categories: , , ,
Product type: Reagents for research
Reagent type: Recombinant protein
Size: 50 µg


The 30kD-secretory protein antigen 85B (Ag85B) is the most abundant protein exposed by Mycobacterium tuberculosis acting as a potent immunoprotective antigen. Ag85B induces a strong T-cell proliferation and the IFN- secretion in most healthy individuals exposed to M. tuberculosis, in BCG-vaccinated mice and humans. It has been shown that antibodies against Ag85B are more prevalent in active tuberculosis patients with decreased cellular immune response.

Additional information

Size 50 µg
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Recombinant protein
Category Mycobacterium tubercolosis
Subcategory Ag85B

Technical information

Accession number UniProtKB/Swiss-Prot: P9WQP0.1
Organism Mycobacterium tuberculosis
Size 50 µg
Expression system E.coli
Tag HIS tagged
Purity >90%
Form Lyophilized. The protein should be reconstituted in apirogenic sterile water or 1X PBS.
Tested application SDS-PAGE, Endotoxin
Shipping/storage conditions Shipped at +RT°C. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately.
Usage For Laboratory Research Use Only



Recombinant bacille Calmette–Guerin coexpressing Ag85b, CFP10, and interleukin-12 elicits effective protection against Mycobacterium tuberculosis

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