Recombinant Listeriolysin O (LLO) is a protein produced in E. coli. LLO is a single polypeptide protein composed of 529 residues lacking the secretion signal peptide (1-25 aa).
Recombinant Listeriolysin-O (LLO)
Description
The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues
Additional information
Size | 50 µg |
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Application | Clinical diagnostics| Veterinary diagnostics| Life Sciences |
Product type | Reagents for research |
Reagent type | Recombinant protein |
Category | Listeria monocytogenes |
Subcategory | Listeriolysin-O |
Technical information
Accession number | GenBank AF253320.1 lacking the secretion signal peptide (1-25aa) |
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Organism | L. monocytogenes |
Size | 50 µg |
Potency | Biologically active protein |
Expression system | E.coli |
Tag | No tags |
Purity | >90% |
Form | Liquid, 50mM NaH2PO4 , 0.5M NaCl, 2.7mM KCl, 1mM EDTA, 1mM DTT, 5%(v/v) glycerol. |
Tested application | WB, SDS-PAGE, Endotoxins, Up-take, Rescue assay |
Shipping/storage conditions | Shipped in dry ice . The protein is stable for 12 months if stored at -80°C. Avoid freeze-thaw cycles. |
Usage | For Laboratory Research Use Only |
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References
A Novel Role of Listeria monocytogenes Membrane Vesicles in Inhibition of Autophagy and Cell Death | PUBMED ID: 28516064 |