Rabbit Anti Listeriolysin O affinity purified

Cholesterol-dependent cytolysins (CDCs) are produced by a large number of pathogenic gram–positive bacteria. A member of this family, listeriolysin O (LLO), is produced by the intracellular pathogen Listeria monocytogenes. Polyclonal anti Listeriolysin antibody affinity purified is designed for the specific detection of LLO in in infected speciment as an useful tool in the research of Listeria monocytogenes infections.The affinity purification protocol garantees an improvement on the specific signal

SKU: ANT0016 Categories: , , ,
Product type: Reagents for research
Reagent type: Antibody
Size: 200 µL

Description

Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a host vacuolar compartment and grows rapidly in the cytosol. Listeriolysin O (LLO), one of its major virulence factors, is a secreted pore-forming protein essential for the escape of L. monocytogenes from the vacuole formed upon initial internalization. In addition, LLO influences the levels of intracellular calcium and stimulates phosphoinositide metabolism to favour bacterial entry. It is also a potent aggregator of lipid rafts and suppresses the host immune response. Phagosomal membrane disruption is enhanced by the bacterial phosphatidylinositol-specific and broad-range phospholipases C, PI-PLC (plcA) and PC-PLC (plcB), respectively. Enzymes cooperate with LLO in the lysis of primary and secondary vacuoles.

Additional information

Size 200 µL
Application Clinical diagnostics| Life Sciences
Product type Reagents for research
Reagent type Antibody
Category Lysteria monocytogenes
Subcategory Polyclonal anti LLO antibody purified against antigen
Antibody type Primary antibody
Immunogen Recombinant purified Listeriolysin O protein expressed in E.coli

Technical information

Accession number GenBank AAP33758.1
Size 200 µL
Purity Purified by affinity Chromatography on Listeriolysin antigen
Form Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.
Shipping/storage conditions Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles.
Usage For Laboratory Research Use Only
Source Rabbit
Specificity Listeriolysin O from infected specimens
React with Listeryolisin O from L. monocytogenes
Recommended dilutions Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values.

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References

A Novel Role of Listeria monocytogenes Membrane Vesicles in Inhibition of Autophagy and Cell Death PUBMED ID: 28516064
Cold-Shock Domain Family Proteins (Csps) Are Involved in Regulation of Virulence, Cellular Aggregation, and Flagella-Based Motility in Listeria monocytogenes PUBMED ID: 29124040

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