Cholesterol-dependent cytolysins (CDCs) are produced by a large number of pathogenic gram–positive bacteria. A member of this family, listeriolysin O (LLO), is produced by the intracellular pathogen Listeria monocytogenes. Polyclonal anti Listeriolysin antibody is designed for the detection of LLO in in infected speciment as an useful tool in the research of Listeria monocytogenes infections.
Rabbit Anti Listeriolysin O
Description
Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a host vacuolar compartment and grows rapidly in the cytosol. Listeriolysin O (LLO), one of its major virulence factors, is a secreted pore-forming protein essential for the escape of L. monocytogenes from the vacuole formed upon initial internalization. In addition, LLO influences the levels of intracellular calcium and stimulates phosphoinositide metabolism to favour bacterial entry. It is also a potent aggregator of lipid rafts and suppresses the host immune response. Phagosomal membrane disruption is enhanced by the bacterial phosphatidylinositol-specific and broad-range phospholipases C, PI-PLC (plcA) and PC-PLC (plcB), respectively. Enzymes cooperate with LLO in the lysis of primary and secondary vacuoles.
Additional information
| Size | 200 µL |
|---|---|
| Application | Clinical diagnostics|Life Sciences |
| Product type | Reagents for research |
| Reagent type | Antibody |
| Category | Lysteria monocytogenes |
| Subcategory | Polyclonal anti LLO antibody |
| Antibody type | Primary antibody |
| Immunogen | Recombinant purified Listeriolysin O protein expressed in E.coli |
Technical information
| Accession number | GenBank AAP33758.1 |
|---|---|
| Size | 200 µL |
| Purity | Purified by Protein A affinity Chromatography |
| Form | Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH. |
| Shipping/storage conditions | Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles. |
| Usage | For Laboratory Research Use Only |
| Source | Rabbit |
| Specificity | Listeriolysin O from infected specimens |
| React with | Listeryolisin O from L. monocytogenes |
| Recommended dilutions | Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values. |
Download
References
| A Novel Role of Listeria monocytogenes Membrane Vesicles in Inhibition of Autophagy and Cell Death | PUBMED ID: 28516064 |
| Cold-Shock Domain Family Proteins (Csps) Are Involved in Regulation of Virulence, Cellular Aggregation, and Flagella-Based Motility in Listeria monocytogenes | PUBMED ID: 29124040 |
