The recombinant Cyclophilin A is a 18 kDa protein with N-terminal His-tag, expressed in E. coli.
Recombinant Cyclophilin A
Description
Cyclophilins belong to the superfamily of immunophilins, highly conserved cytoplasmic proteins which share the PPI enzymatic activity and the ability to bind immunosuppressive drugs. CypA binds to and mediates the effect of Cyclosporin A; the CsA/CypA complex selectively binds and inactivates the serine/theronine phosphatase calcineurin leading to disruption of T-cells activating cascade. CsA binding occurs at the active site of the protein thus inhibiting its enzymatic activity. CypA has been demonstrated to be involved in several steps of viral infections such as those mediated by HIV, HBV and HCV.
Cyclophilin A (CypA, 18 kDa) is the first peptidyl-prolil cis-trans isomerase (PPIase) to be discovered; PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins.
Additional information
| Size | 500 µg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Recombinant protein |
| Category | Cyclophilins |
Technical information
| Accession number | GenBank NM_021130.3 |
|---|---|
| Organism | Homo sapiens peptidylprolyl isomerase A (cyclophilin A) (PPIA), mRNA |
| Size | 500 µg |
| Expression system | E.coli |
| Tag | HIS tagged |
| Purity | >99% |
| Form | Liquid, 20mM Tris pH 8.0, 20mM NaCl, 0.5mM DTT, 10%(v/v) glycerol. |
| Tested application | SDS-PAGE/WB |
| Shipping/storage conditions | Shipped at -20°C. The protein is stable for 12 months if stored at -80°C. Avoid repeated freeze/thaw cycles. |
| Usage | For Laboratory Research Use Only |
