DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

STEC Serotypes FLUO kit


STEC Serotypes FLUO kit consente l’identificazione dei sierotipi di Escherichia coli produttori di Shiga tossine (STEC) precedentemente risultati positivi ai geni stx e/o eae (STEC FLUO Detection kit-MBK0068, Diatheva). Il kit è stato sviluppato in accordo con i requisiti specificati nella norma ISO/TS 13136:2012 ed il metodo USDA MLG 5B.02.



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Info

Contenuto del kit:

Master mix O26-O103: 2 x 250 μl
Master mix O145-O104: 2 x 250 μl
Master mix O157-O111: 2 x 250 μl
Hot Rescue DNA Polymerase (5U/μl): 1 x 15 μl
DNAse free water: 1 x 1000 μl
Positive Control: 1 x 100 μl
ROX: 6 x 5 μl
Dilution Buffer: 1 x 2000 μl

Papers

 

-Choreh Farrokh, Kieran Jordan, Frederic Auvrayc, Kathleen Glass,, Hanne Oppegaard, Sabrina Raynaud, Delphine Thevenot, Robin Condron, Koen De Reu Alexander Govaris Klaus Heggum Marc Heyndrickx Joerg Hummerjohann Denise Lindsay Stephane Miszczycha Sylvie Moussiegt Karen Verstraetei Olivier Cerf, 2013. Review of Shiga-toxin-producing Escherichia coli (STEC) and their significance in dairy production, International Journal of Food Microbiology Volume 162, Issue 2.

-ISO/TS 13136, 2012. Microbiology of food and animal feed-Real-Time polymerase chain reaction (PCR) - based method for the detection of food-borne pathogens – Horizontal method for the detection of Shiga toxin producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups.

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