The kit is qPCR assay to detect Enteroaggregative E. coli by the amplification of a specific sequences of two genetic markers of typical EAEC: the plasmid located gene aggR, coding for a transcription regulator, and the chromosomal gene aaiC, which is part of the aai gene cluster, encoding a type VI secretion system, with the use of fluorescent-labelled probe.
The kit provides a ready to use PCR mix containing an Internal Control to assess the efficiency of amplification reaction, revealing the presence of inhibitory factors in the sample. The Enteroaggregative E. coli kit also contains a PCR Positive Control.
Enteroaggregative E.Coli Detection Kit
Description
Enteroaggregative Escherichia coli has been cited as an important childhood enteric pathogen worldwide (Jensen et al., 2014). A recent large and lethal German outbreak caused by a Shiga toxin (Stx)-producing EAEC, brought attention to this pathogen that did not possess the eae gene, encoding the adherence factor intimin, but possessed genes that are typical of the EAEC group (Scheutz et al., 2011). This matter has highlighted the importance to screen foodstuffs for EAEC too by the detection of targets designed on the aggR and aaiC genes, which represent genetic markers characteristic for this group of pathogenic E. coli (EFSA, 2013).
Additional information
| Size | 50 tests |
|---|---|
| Application | Food Science |
| Product type | Diagnostic kit |
| Reagent type | PCR |
| Category | Foodborne pathogens |
Technical information
| Size | 50 tests |
|---|---|
| Technology | Real-time PCR |
| Specimen | Bacterial DNA isolated from food and environmental samples |
| Testing time | 2 hours |
