Microtiter strips coated with LLO antigen are incubated with collected samples. During this incubation step, anti-LLO antibodies bind to the antigen, forming specific complexes. Antigen-antibody complexes are detected by anti-ruminant IgG HRP-conjugated secondary antibody. Revelation step is performed incubating the strips with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as chromogen and reading the absorbance values at 410 ±5 nm by ELISA microplate reader.
Listeria Goat Test
Description
Listeria monocytogenes is the causative agent of listeriosis, a severe nervous disease associated with a high case fatality rate. Among the domestic animals, the disease most commonly occurs in ruminants [Cooper J & Walker RD, 1998]. Listeriolysin O (LLO), is a major virulence factor and it was confirmed that it can induces a strong humoral response during infection, even when animals were infected with subclinical infecting doses of L. monocytogenes [Lhopital et al., 1993].
Additional information
| Size | 96 tests/ 1 plate |
|---|---|
| Application | Veterinary diagnostics |
| Product type | Diagnostic kit |
| Reagent type | ELISA |
| Category | Goat |
Technical information
| Size | 96 tests/ 1 plate |
|---|---|
| Technology | Indirect ELISA |
| Specimen | Goat serum samples |
| Testing time | 3 hours |
