Listeria Goat Test

Microtiter strips coated with LLO antigen are incubated with collected samples. During this incubation step, anti-LLO antibodies bind to the antigen, forming specific complexes. Antigen-antibody complexes are detected by anti-ruminant IgG HRP-conjugated secondary antibody. Revelation step is performed incubating the strips with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as chromogen and reading the absorbance values at 410 ±5 nm by ELISA microplate reader.

SKU: AKE0005 Categories: , ,
Application: Veterinary diagnostics
Product type: Diagnostic kit
Reagent type: ELISA
Size: 96 tests/ 1 plate

Description

Listeria monocytogenes is the causative agent of listeriosis, a severe nervous disease associated with a high case fatality rate. Among the domestic animals, the disease most commonly occurs in ruminants [Cooper J & Walker RD, 1998]. Listeriolysin O (LLO), is a major virulence factor and it was confirmed that it can induces a strong humoral response during infection, even when animals were infected with subclinical infecting doses of L. monocytogenes [Lhopital et al., 1993].

Additional information

Size 96 tests/ 1 plate
Application Veterinary diagnostics
Product type Diagnostic kit
Reagent type ELISA
Category Goat

Technical information

Size 96 tests/ 1 plate
Technology Indirect ELISA
Specimen Goat serum samples
Testing time 3 hours

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