The Listeria monocytegenes FLUO Kit is intended for the detection of Listeria monocytegenes in food and environmental samples. The kit provide an easy-to-use master mix and contains reagent, enzyme and positive control for the successful amplification and detection of DNA from Listeria monocytogenes in multiplex Real-Time PCR using dual-labelled probes.
Listeria monocytogenes FLUO Kit
Intoxications and infections caused by foodborne pathogens represent an increasing public health problem, the fast and accurate identification of L. monocytogenes from food samples by Public health agencies and diagnostic laboratories insures not only a better quality of products, but also the possibility to adopt timely precautionary measures to limit the spread of infection in case of an outbreak. The Listeria monocytogenes FLUO kit uses DNA primers and fluorescent probe specific for the target organism. The internal control, present in the amplification mix, assesses the efficiency of amplification reaction by checking the presence of inhibitory factors and ensuring reliability of negative results. Results are obtained within a few hours following an enrichment step and subsequent DNA extraction, assuring 100% specificity and 1 CFU after enrichment sensitivity.
|Bacterial DNA isolated from food and environmental samples