Grains DNA Extraction Kit

Grains DNA extraction kit allows the isolation of genomic DNA from corn, flour and derivative products such as pasta.

SKU: MBK0064 Categories: , ,
Application: Food Science
Product type: Diagnostic kit
Reagent type: DNA Isolation
Size: 50 tests

Description

The composition in cereals of specific foods is a key factor for the quality of the final product. The cereal of choice for the production of pasta is durum wheat due to its special features such as colour and protein content, that affect the quality of pasta. Grains DNA extraction kit is formulated for the DNA isolation from pasta and flours in order to verify, through the use of Grain quantitative kit (Diatheva, MBK0062), the content of soft wheat in the final product caused by cross contaminations that may occur during growing, harvesting and handling practices of durum wheat. The Grains DNA Extraction Kit combines an efficient lysis process with a purification system, using a binding buffers in combination with columns. The silica membrane was optimized for an high yield of DNA and low affinity for the binding of impurities. After homogenization of the sample, is added the GL Lysis buffer containing chaotropic salts, denaturing agents and detergents that ensure optimal lysis action on the sample which is then clarified by centrifugation. The add of GB Binding buffer creates the optimal conditions for the binding of DNA to the silica membrane of the column. The impurities are removed with a simple washing, and the purified DNA was eluted in a low ionic strength buffer.

Additional information

Size 50 tests
Application Food Science
Product type Diagnostic kit
Reagent type DNA Isolation
Category Ingredient authentication

Technical information

Size 50 tests
Technology Spin-column based DNA extraction
Specimen Corn, flour and derivative products such as pasta
Testing time 2 hours

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References

Related products

Grain Quantitative Kit
The Grain Quantitative kit allows a reliable quantification of soft wheat adulteration in durum wheat-based foodstuffs. The method is based on the quantification of Triticum aestivum in Triticum spp. by Real-Time PCR.
MBK0062

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