Grains DNA Extraction Kit

Description

The composition in cereals of specific foods is a key factor for the quality of the final product. The cereal of choice for the production of pasta is durum wheat due to its special features such as colour and protein content, that affect the quality of pasta. Grains DNA extraction kit is formulated for the DNA isolation from pasta and flours in order to verify, through the use of Grain quantitative kit (Diatheva, MBK0062), the content of soft wheat in the final product caused by cross contaminations that may occur during growing, harvesting and handling practices of durum wheat. The Grains DNA Extraction Kit combines an efficient lysis process with a purification system, using a binding buffers in combination with columns. The silica membrane was optimized for an high yield of DNA and low affinity for the binding of impurities. After homogenization of the sample, is added the GL Lysis buffer containing chaotropic salts, denaturing agents and detergents that ensure optimal lysis action on the sample which is then clarified by centrifugation. The add of GB Binding buffer creates the optimal conditions for the binding of DNA to the silica membrane of the column. The impurities are removed with a simple washing, and the purified DNA was eluted in a low ionic strength buffer.