The Grain Quantitative kit allows a reliable quantification of soft wheat adulteration in durum wheat-based foodstuffs. The method is based on the quantification of Triticum aestivum in Triticum spp. by Real-Time PCR.
Grain Quantitative Kit
The Grain quantitative kit can be used to determine the relative DNA copy number ratio of Triticum aestivum in Triticum spp. by comparing the amplification of a specific gene of Triticum aestivum (soft grain) with normaliser gene common of all species of Triticum genus. The relative quantification is obtained by the use of two calibration curves, one for each target gene. The kit contains a Standard DNA for the preparation of the calibration curves, containing DNA of Triticum turgidum and aestivum. The Standard DNA supplied with the kit is derived from Triticum aestivum and Triticum durum (Triticum turgidum) certified seeds. The kit include also the 3% DNA solution, a ready-to-use DNA solution that contains 3±0.9% of Triticum aestivum/Triticum spp. prepared from the same certified seeds used for the preparation of the Standard curve.
|Corn, flour and derivative products such as pasta