Recombinant (HIV-1) Tat clade D produced in E. coli. Productive HIV-1 replication is absolutely dependent upon the viral tat protein. In Uganda Clades A and D are responsible for approximately 95% of HIV-1 infections. The HIV-1 tat protein Clade A is a molecule of 86 aminoacids encoded by two exons and having a molecular mass of 18 kDa in SDS-PAGE.
Recombinant TAT Clade D
HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).
HIV-1 regulatory tat protein plays an essential role in viral replication and infectivity. In addition, during acute infection tat is released in the extracellular environment by infected cells and is taken up by neighbouring cells where it transactivates viral replication and increases virus infectivity. The HIV-1 tat protein is a molecule of 86 aminoacids encoded by two exons.
|Clinical diagnostics| Life Sciences
|Reagents for research
|TAT Protein (Transactivating regulatory protein)
|HIV-1 isolate UG28R from Uganda tat protein (tat) gene, partial cds. Sequence conservation and antibody cross-recognition of clade B human immunodeficiency virus (HIV) type 1 Tat protein in HIV-1-infected Italians, Ugandans, and South Africans J. Infect. Dis. 188 (8), 1171-1180 (2003)
|Not biologically Active protein
|Lyophilized with 10% glycerol.
|WB, SDS-PAGE, Endotoxins, Up-take, Rescue assay
|The protein should be reconstituted in apirogenic sterile water or 1X PBS
|Shipped at RT. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.
|For Laboratory Research Use Only