HIV-1 TAT Recombinant- produced in E.coli is a single, non-glycosylated, polypeptidic chain containing 86 amino acids encoded by two exons and having a molecular mass of 14kDa.
Recombinant TAT Clade B
Tat is a key viral regulatory protein produced very early after infection, even prior to HIV integration, and necessary for reverse transcription (Lalonde, 2011), viral gene expression (Arya, 1985; Fisher, 1986; Ensoli, 1993; Wu, 2001), cell-to-cell virus transmission, virus dissemination and disease progression. In fact, in the absence of Tat, no or negligible amounts of structural proteins are expressed and, therefore, no infectious virus progeny is made produced.
Tat is released extracellularly and accumulates in tissues where it increases virus infectivity and activates immune cells while disabling the immune response to HIV (Ensoli, 1990, 1993, 1994, Ott, 1997; Chang, 1997)
HIV-1 regulatory Tat protein plays an essential role in viral replication and infectivity. In addition, during acute infection tat is released in the extracellular environment by infected cells and is taken up by neighbouring cells where it transactivates viral replication and increases virus infectivity. The HIV-1 tat protein is a molecule of 86 aminoacids encoded by two exons.
|Application||Clinical diagnostics| Life Sciences|
|Product type||Reagents for research|
|Reagent type||Recombinant protein|
|Subcategory||TAT Protein (Transactivating regulatory protein)|
|Accession number||UniProt P69697.1|
|Organism||Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)|
|Potency||Biologically active protein estimated by Up-Take and Rescue assays (positive to both assays)|
|Form||Lyophilized with 0.1% glycerol.|
|Tested application||WB, SDS-PAGE, Endotoxins, Up-take, Rescue assay|
|Solubility||The protein should be reconstituted in apirogenic sterile water or 1X PBS|
|Shipping/storage conditions||Shipped in dry ice . The lyophilized protein is stable for 24 months if stored at -80°C. The reconstituted solution has to be used immediately. Avoid freeze-thaw cycles.|
|Usage||For Laboratory Research Use Only|
|Continued Decay of HIV Proviral DNA Upon Vaccination With HIV-1 Tat of Subjects on Long-Term ART: An 8-Year Follow-Up Study||PUBMED ID: 30815001|
|Mutational effects of human dopamine transporter at tyrosine88, lysine92, and histidine547 on basal and HIV-1 Tat-inhibited dopamine transport||PUBMED ID: 30846720|
|Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses||PUBMED ID: 31337802|
|The role of human dopamine transporter in NeuroAIDS||PUBMED ID: 28987321|
|Differential mechanisms of inflammation and endothelial dysfunction by HIV-1 subtype-B and recombinant CRF02_AG Tat proteins on human brain microvascular endothelial cells: implications for viral neuropathogenesis.||PUBMED ID: 28127697|