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Anti Photobacterium damselae piscicida Igs

Enzyme linked immunosorbent assay (ELISA) for the qualitative determination of anti-Php presence in Fish serum as an aid in the diagnosis of antibody production in Fish infected by Photobacterium damselae subsp. Piscicida. (...more...)

Sample or standards are pipetted directly into wells of a microplate pre-coated with Php inactivated bacteria and pre-blocked , that can capture anti- Igs thereby immobilizing to the well.


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(...more...) This assay is based on the microplate enzyme immunoassay technique, The Php inactivated bacteria have been coated onto the wells of the plate. Revelation step includes an polyclonal anti -Igs HRP-conjugated specific for fish developed in Rabbit and ABTS as chromogen.

"Photobacterium damselae subspecies piscicida" is an important pathogen of many fish species cultured in brackish water in the United States, Japan, Europe and the Mediterranean. Photobacterium damselae subsp. piscicida is a fish pathogen which causes serious diseases in commercial warmwater fish species, infact Photobacteriosis causes severe losses in cultured yellowtail juveniles, and mortalities in other cultured fishes, including ayured sea bream, black sea bream, oval file fish and red grouper. The disease has great economic impact both in Japan, where it affects mainly yellowtail cultures, and in the Mediterranean area, due to the losses , it causes in seabream and seabass farms. The pathogen was studied morphologically, physiologically, and serologically by Jansen and Surgalla (1968), who proposed the name Pasteurella piscicida. Based on rRNA sequence and DNA-DNA hybridization studies, the organism was renamed Photobacteriumdamselae subsp. piscicida, and then corrected to Photobacterium damselae subsp. piscicida . The complications associated to the chemotherapy in disease control avoid their use in Photobacteriosis therapy, the immunization strategy have been proposed as optimal method to control the pathology.


Limitations of the procedure:


• No drugs have been investigated for assay interference
• The kit should not be used beyoind the expiration date
• Any variation in specimen diluent, incubation time or temperature can cause variation in binding.



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