The specific antibodies against different subunits of spike (S) protein of SARS-CoV-2 are excellent research tools for the research against coronavirus. Anti SARS CoV-2 Spike1 monoclonal antibody detects full-length Spike1 also in the glycosylated form. The antibody does not cross react with SARS CoV-2 Spike2 and SARS CoV-2 Spike1 RBD domain.
SARS-CoV-2 Spike S1
Description
Coronavirus disease 2019 is a newly emerging infectious disease currently spreading across the world. It is caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The spike (S) protein of SARS-CoV-2, which plays a key role in the receptor recognition and cell membrane fusion process, is composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain that recognizes and binds to the host receptor angiotensin-converting enzyme 2, while the S2 subunit mediates viral cell membrane fusion by forming a six-helical bundle via the two-heptad repeat domain. The specific antibodies against different subunits of spike (S) protein of SARS-CoV-2 are excellent research tools for the research against coronavirus
Additional information
| Size | 100 μg |
|---|---|
| Application | Clinical diagnostics| Life Sciences |
| Product type | Reagents for research |
| Reagent type | Antibody |
| Category | Covid-19 |
| Subcategory | Monoclonal antibody |
| Antibody type | Primary antibody |
| Immunogen | Conformational SARS-CoV-2 Spike 1 |
Technical information
| Size | 100 μg |
|---|---|
| Purity | Mouse monoclonal immunoglobulins IgG2b subclass were purified by protein A affinity chromatography and stabilized with 0.05% of glycerol. |
| Form | Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH. |
| Shipping/storage conditions | Shipped at -20°C When stored at -20°C, the antibody is stable for 12 months. |
| Usage | For Laboratory Research Use Only |
| Source | mouse |
| Specificity | Anti SARS CoV-2 Spike1 monoclonal antibody detects full-length Spike1 also in the glycosylated form. The antibody does not cross react with SARS CoV-2 Spike2 and SARS CoV-2 Spike1 RBD domain. |
| React with | Conformational SARS-CoV-2 Spike 1 |
| Additional notes | When using any primary antibody or fluorescence-labelled secondary antibody for the first time, titrate out the antibody to determine which dilution allows the strongest specific signal with the lowest background for your sample. |
| Recommended dilutions | Recommended starting dilutions can vary lot-to-lot. |
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References
| Clinical and Analytical Performance of an Automated Serological Test That Identifies S1/S2-Neutralizing IgG in COVID-19 Patients Semiquantitatively. | doi: 10.1128/JCM.01224-20 |
| Structures and distributions of SARS-CoV-2 spike proteins on intact virions. | doi: 10.1038/s41586-020-2665-2 |
| SARS-CoV-2-specific virulence factors in COVID-19. | doi: 10.1002/jmv.26615 |
