The recombinant Ag85B is a 325amino acids protein with a molecular weight of about 30 kDa and is produced in E.coli.
Recombinant Mycobacterium tuberculosis Ag85B
The 30kD-secretory protein antigen 85B (Ag85B) is the most abundant protein exposed by Mycobacterium tuberculosis acting as a potent immunoprotective antigen. Ag85B induces a strong T-cell proliferation and the IFN- secretion in most healthy individuals exposed to M. tuberculosis, in BCG-vaccinated mice and humans. It has been shown that antibodies against Ag85B are more prevalent in active tuberculosis patients with decreased cellular immune response.
|Application||Clinical diagnostics| Life Sciences|
|Product type||Reagents for research|
|Reagent type||Recombinant protein|
|Accession number||UniProtKB/Swiss-Prot: P9WQP0.1|
|Form||Lyophilized. The protein should be reconstituted in apirogenic sterile water or 1X PBS.|
|Tested application||SDS-PAGE, Endotoxin|
|Shipping/storage conditions||Shipped at +RT°C. The lyophilized protein is stable for 24 months if stored at -20°C. The reconstituted solution has to be used immediately.|
|Usage||For Laboratory Research Use Only|
|Recombinant bacille Calmette–Guerin coexpressing Ag85b, CFP10, and interleukin-12 elicits effective protection against Mycobacterium tuberculosis|