The PBRT 2.0 Kit is intended for molecular typing of plasmid conferring drug resistance in Enterobacteriaceae.
PBRT 2.0 Kit
Description
The PBRT 2.0 system is a PCR-based assay that allows fast and easy plasmid characterization and molecular identification through replicons detection. Together with a convenient format the system represents a valid tool to test a wide panel of bacterial strains and has been extensively used to classify plasmids, following the dissemination of resistance determinants in different countries and environments (Carattoli A. et al., 2009).
Additional information
| Size | 192 tests |
|---|---|
| Application | Antimicrobial resistance |
| Product type | Diagnostic kit |
| Reagent type | PCR |
Technical information
| Size | 192 tests |
|---|---|
| Technology | End-point PCR |
| Specimen | Veterinary, clinical or food samples |
| Testing time | 1.5 hour |
| Target gene | HI1, HI2, I1, I2, X1, X2, X3, X4, L, M, N, FIA, FIB, FIC, FII, FIIS, FIIK, FIB KN, FIB, KQ, W, Y, P1, A/C, T, K, U, R, B/O, HIB-M and FIB-M |
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References
| Identification of plasmids by PCR-based replicon typing. | PMID: 15935499 |
| Resistance plasmid families in Enterobacteriaceae | PMID: 19307361 |
| Identification and classification of bacterial plasmids | PMID: 3054468 |
| Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella | PMID: 19001452 |
| Klebsiella pneumoniae ST258 producing KPC-3 identified in italy carries novel plasmids and OmpK36/OmpK35 porin variants | PMID: 22252815 |
| Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants | PMID: 20935300 |
| Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes. J. Antimicrob | PMID: 22511638 |
