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PBRT 2.0 kit - New replicons (P1, N2, FIB-KN, FIB-KQ, X4)

The PBRT 2.0 kit is intended for molecular typing of plasmid conferring drug resistance in Enterobacteriaceae.


The epidemiology of resistance plasmids is a major issue for the description of antimicrobial resistance diffusion. Plasmids can be categorized in families on the basis of their phylogenetic relatedness.  The molecular identification and classification of plasmids by PCR is currently possible and it is based on the detection of plasmid replication controls (replicons), namely the portion of large, naturally occurring plasmids encoding the replicase gene (rep) and cis-acting regulatory elements, controlling plasmid DNA replication and copy number. 


The PBRT 2.0 system is a PCR-based assay that allows fast and easy plasmid characterization and molecular identification through replicons detection. Together with a convenient format the system represents a valid tool to test a wide panel of bacterial strains and has been extensively used to classify plasmids, following the dissemination of resistance determinants in different countries and environments (Carattoli A. et al., 2009).


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Price:
€ 450,00

PAPERS

Occurrence of Extended Spectrum β-Lactamases, KPC-Type, and MCR-1.2-Producing Enterobacteriaceae from Wells, River Water, and Wastewater Treatment Plants in Oltrepò Pavese Area, Northern Italy

A Novel IncA/C1 Group Conjugative Plasmid, Encoding VIM-1 Metallo-Beta-Lactamase, Mediates the Acquisition of Carbapenem Resistance in ST104 Klebsiella pneumoniae Isolates from Neonates in the Intensive Care Unit of V. Monaldi Hospital in Naples

VIM-1 carbapenemase-producing Escherichia coli isolated from retail seafood, Germany 2016

Outbreak of colistin-resistant carbapenemase-producing Klebsiella pneumoniae in Tunisia

NDM-5 and OXA-181 beta-lactamases: a significant threat continues to spread in the Americas

ST147 NDM-1-producing Klebsiella pneumoniae spread in two Tunisian hospitals

Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango

ST48 Escherichia coli carrying the blaCTX-M-1 IncI1/ST3 plasmid in drinking water, France

Extended-spectrum β-lactamase-producing Salmonella enterica serovar Infantis from humans in Italy

Unexpected common occurrence of transferable extended spectrum cephalosporinase-producing Escherichia coli in Swedish surface waters used for drinking water supply

High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain

Cephalosporin-resistant Escherichia coli in the Norwegian broiler production pyramid -genetic characterization and determination of risk factors

Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain

OXA-48-producing ST372 Escherichia coli in a French dog

Diversity, virulence, and antimicrobial resistance of the KPCproducing Klebsiella pneumoniae ST307 clone

Spread and exchange of blaNDM-1 in hospitalized neonates: role of mobilizable genetic elements

Intestinal colonisation with extended-spectrum cephalosporin- and colistin-resistant Enterobacteriaceae in HIV-positive individuals in Switzerland: molecular features and risk factors

Comparative analysis of the standard PCR-Based Replicon Typing (PBRT) with the commercial PBRT-KIT

Spread and exchange of blaNDM-1 in hospitalized neonates: role of mobilizable genetic elements

Intestinal colonisation with extended-spectrum cephalosporin-resistant Escherichia coli in Swiss pets: molecular features, risk factors and transmission with owners

Host-Specific Patterns of Genetic Diversity among IncI1-I gamma and IncK Plasmids Encoding CMY-2 beta-Lactamase in Escherichia coli Isolates from Humans, Poultry Meat, Poultry, and Dogs in Denmark

Emergence of the colistin resistance mcr-1 determinant in commensal Escherichia coli from residents of long-term-care facilities in Italy

The colistin resistance mcr-1 gene is going wild

First description of plasmid-mediated colistin-resistant extended-spectrum β-lactamase-producing Escherichia coli in a wild migratory bird from Asia

Absence of co-localization between pathovar-associated virulence factors and extended-spectrum β-lactamase (blaCTX-M) genes on a single plasmid

Coexistence of mcr-1 and blaNDM-1 in Escherichia coli from Venezuela

Diverse distribution of Toxin-Antitoxin II systems in Salmonella enterica serovars

Travelers Can Import Colistin-Resistant Enterobacteriaceae Including Those Possessing the Plasmid-Mediated mcr-1 Gene

Plasmid and Host Strain Characteristics of Escherichia coli Resistant to Extended-Spectrum Cephalosporins in the Norwegian Broiler Production

Characterization of tet(Y)-carrying LowGC plasmids exogenously captured from cow manure at a conventional dairy farm

Limited Dissemination of Extended-Spectrum β-Lactamase– and Plasmid-Encoded AmpC–Producing Escherichia coli from Food and Farm Animals, Sweden

Emergence of a Clonal Lineage of Multidrug-Resistant ESBL-Producing Salmonella Infantis Transmitted from Broilers and Broiler Meat to Humans in Italy between 2011 and 2014

High prevalence of international ESBL CTX-M-15-producing Enterobacter cloacae ST114 clone in animals

Providencia stuartii Isolates from Greece: Co-Carriage of Cephalosporin (blaSHV-5, blaVEB-1), Carbapenem (blaVIM-1), and Aminoglycoside (rmtB) Resistance Determinants by a Multidrug-Resistant Outbreak Clone

Emergence of Escherichia coli Sequence Type 131 (ST131) and ST3948 with KPC-2, KPC-3 and KPC-8 carbapenemases from a Long-Term Care and Rehabilitation Facility (LTCRF) in Northern Italy

Double copies of Tn4401a:blaKPC-3 on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy

Molecular characterization of bacteremic Escherichia coli isolates in Romania

In vivo Evolution of CMY-2 to CMY-33 β-Lactamase in Escherichia coli ST131: Characterization of an Acquired Extended-Spectrum AmpC (ESAC) Conferring Resistance to Cefepime.

Molecular Analysis of Antibiotic Resistance Determinants and Plasmids in Malaysian Isolates of Multidrug Resistant Klebsiella pneumoniae

Extended-Spectrum-Beta-Lactamases, AmpC Beta-

Occurrence and Genetic Characteristics of Third-Generation Cephalosporin-Resistant Escherichia coli in Swiss Retail Meat

Extended-Spectrum-Beta-Lactamases, AmpC Beta-Lactamases and Plasmid Mediated Quinolone Resistance in Klebsiella spp. from Companion Animals in Italy

Enterobacteriaceae resistant to third-generation cephalosporins and quinolones in fresh culinary herbs imported from Southeast Asia

High diversity of plasmids harbouring blaCMY-2 among clinical Escherichia coli isolates from humans and companion animals inthe upper Midwestern USA

Rapid plasmid replicon typing by real time PCR

Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland

Cefotaxime Resistant Escherichia coli Collected from a Healthy Volunteer; Characterisation and the Effect of Plasmid Loss

USE OF ANTIMICROBIALS AND CEPHAMICIN RESISTANCE IN COMPANION ANIMALS

Detection of antibiotic residues and association of cefquinome

CMY-2-Producing Escherichia coli in the Nose of Pigs

Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland

Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK v

Klebsiella pneumoniae ST11 from companion animals bearing ArmA methyltransferase, DHA-1 β-lactamase and QnrB4

blaCTX-M-15-carrying Escherichia coli and Salmonella isolates from livestock and food in Germany

INFO

The PBRT system is a PCR-based assay that allows fast and easy plasmid characterization and molecular identification through replicons detection (Carattoli et al. 2005). Together with a convenient format the system represents a valid tool to test a wide panel of bacterial strains and has been extensively used to classify plasmids, following the dissemination of resistance determinants in different countries and environments (Carattoli A. et al., 2009).

Principle of the assay: The PBRT kit provides a novel set of 8 specific standard PCR assays optimized to perform 8 multiplex PCRs for the amplification of 25 replicons: HI1, HI2, I1, I2, X1, X2, L/M, N, FIA, FIB, FIC, FII, FIIS, FIIK, W, Y, P, A/C, T, K, U, R, B/O, HIB-M and FIB-M, representative of major plasmid incompatibility groups and replicase genes identified on resistance plasmids circulating among Enterobacteriaceae. Positive controls for all the respective replicons are included in the kit.

 

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