DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

DI-Check Legionella pneumophila kit

The DI-Check Legionella pneumophila kit is intended for the detection and/or quantification of L. pneumophila. The assay is based on dual-labelled real-time PCR technology. Dye-labeled probes target unique DNA sequences specific to L. pneumophila and synthetic internal amplification control DNA (IAC). Target DNA, if present, is amplified by PCR and detected in real-time using fluorescent hydrolysis probe chemistry. The fluorescence signal that is generated is detected by the real-time PCR instrument and interpreted by the analysis software.
The kit provides a ready to use PCR mix highly resistant to PCR inhibitors and with an extremely stringent automatic hot-start allowing reaction assembly and temporary storage at room temperature prior to PCR amplification. This robust PCR mix contains an internal control to assess the efficiency of amplification reaction revealing the presence of inhibitory factors in the sample. Quantification of the target Legionella species is carried out based on a standard curve covering a concentration range of 25000–25 genome units (GU) per PCR reaction.


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Price:
€ 550,00
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