DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Multipathogen FLUO kit

Multipathogen FLUO kit represents an alternative PCR-based approach for the qualitative concurrent detection of the cited bacterial species. The kit uses DNA primer and fluorescent probes specific for the target organisms. If pathogens are present, DNAs are amplified and the increased fluorescence signals are recordered in Real-Time. The internal control, presents in the amplification mix, assesses the efficiency of each amplification reaction by checking the presence of inhibitory factors and ensuring reliability of negative results.

The kit could be used for the simultaneous identification of pathogens: Salmonella spp., L.monocytogenes and E.coli O157, by multiplex Real-time PCR.

Kit Contents:

- 2 x 0.5 ml 1X Master mix
- 1 x 15 μl 5U/μl DNA polymerase
- 1 x 0.2 ml Positive control (105 cells for each target species/5 μl)
- 1 x 1 ml PCR Grade water

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Intoxications and infections caused by food-borne pathogens represent an increasing public health problem, with nearly a quarter of the population at higher risk for illness today (Oliver et al., 2005). Several outbreaks of food-borne illnesses following consumption of several food caused by Shiga toxin-producing E.coli, Salmonella spp. and L.monocytogenes have been reported in recent years, indicating the importance of this problem in safeguarding public health. Particularly, numerous outbreaks of disease were related to consumption of unpasteurized milk and derived dairy products (Le jeune, 2009). The fast and accurate identification of microbial pathogens from food samples by Public health agencies and diagnostic laboratories insures not only a better quality of products, but also the possibility to adopt timely precautionary measures to limit the spread of infection in case of an outbreak. Conventional assays in common use can take up to days or more.


Multipathogen FLUO kit provides an easy-to-use mastermix dedicated for the use with the instruments with four-plex capability. The kit contains reagent, enzyme and positive control for the successful amplification and detection of DNA from Salmonella spp., L.monocytogenes and E.coli O157 in multiplex real-time PCR using dual-labelled probes. Up to 4 genes (1 control gene and 3 target genes) can be detected simultaneously in the same reaction.

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