DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Enteroaggregative E. coli detection kit

The Enteroaggregative E. coli kit is qPCR assay to detect Enteroaggregative E. coli by the amplification of a specific sequences of two genetic markers of typical EAEC: the plasmid located gene aggR, coding for a transcription regulator, and the chromosomal gene aaiC, which is part of the aai gene cluster, encoding a type VI secretion system, with the use of fluorescent-labelled probe.
The kits provide a ready to use PCR mix containing anInternal Control to assess the efficiency of amplification reaction revealing the presence of inhibitory factors in the sample. The Enteroaggregative E. coli kit also contains Positive PCR Control.


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Kit contents:

1 x 400μl Enteroaggregative E. coli PCR mix 
1 x 5 μl Hot rescue DNA polymerase 5U/μl
1 x 5 μl ROX solution
1 x 100 μl Dilution Buffer
1 x 50 μl Positive PCR Control (aggR and aaiC)
1 x 100 μl Negative PCR Control


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