Hot Rescue PLUS DNA Polymerase

Hot Rescue PLUS DNA Polymerase is a hot-start highly thermostable Taq which catalyzes 5’-3’ synthesis of DNA with no detectable 3’-5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning. The enzyme is provided at a concentration of 5 U/µL with 25 mM Magnesium Chloride solution and 10X Reaction Buffer.

SKU: MBR0001-P Categories: , ,
Application: Life Sciences
Product type: Reagents for research
Reagent type: PCR
Size: 250 U

Description

Hot Rescue PLUS DNA Polymerase is a chemically modified HotStart DNA polymerase which has no activity below 74 °C and requires a thermal activation of 10 minutes at 95 °C to reach maximal activity, avoiding non-specific priming at low temperature during the PCR set-up. Hot Rescue PLUS DNA Polymerase is more heat-stable than commonly used enzymes and is particularly recommended for PCR applications that require ultra low levels of DNA and for amplification of templates prone to produce unspecific amplicons.

Additional information

Size 250 U
Application Life Sciences
Product type Reagents for research
Reagent type PCR
Category Molecular Biology

Technical information

Size 250 U
Technology Real-time PCR

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