Hot Rescue PLUS DNA Polymerase is a hot-start highly thermostable Taq which catalyzes 5’-3’ synthesis of DNA with no detectable 3’-5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning. The enzyme is provided at a concentration of 5 U/µL with 25 mM Magnesium Chloride solution and 10X Reaction Buffer.
Hot Rescue PLUS DNA Polymerase
Hot Rescue PLUS DNA Polymerase is a chemically modified HotStart DNA polymerase which has no activity below 74 °C and requires a thermal activation of 10 minutes at 95 °C to reach maximal activity, avoiding non-specific priming at low temperature during the PCR set-up. Hot Rescue PLUS DNA Polymerase is more heat-stable than commonly used enzymes and is particularly recommended for PCR applications that require ultra low levels of DNA and for amplification of templates prone to produce unspecific amplicons.
|Product type||Reagents for research|