Hot Rescue PLUS DNA Polymerase is a hot-start highly thermostable Taq which catalyzes 5’-3’ synthesis of DNA with no detectable 3’-5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning. The enzyme is provided at a concentration of 5 U/µL with 25 mM Magnesium Chloride solution and 10X Reaction Buffer.
Hot Rescue PLUS DNA Polymerase
Description
Hot Rescue PLUS DNA Polymerase is a chemically modified HotStart DNA polymerase which has no activity below 74 °C and requires a thermal activation of 10 minutes at 95 °C to reach maximal activity, avoiding non-specific priming at low temperature during the PCR set-up. Hot Rescue PLUS DNA Polymerase is more heat-stable than commonly used enzymes and is particularly recommended for PCR applications that require ultra low levels of DNA and for amplification of templates prone to produce unspecific amplicons.
Additional information
| Size | 250 U |
|---|---|
| Application | Life Sciences |
| Product type | Reagents for research |
| Reagent type | PCR |
| Category | Molecular Biology |
Technical information
| Size | 250 U |
|---|---|
| Technology | Real-time PCR |
