Custom protein production service
We offer three different packages to produce your desired protein
PACKAGE 1
FROM SEQUENCE TO PROTEIN
- PACKAGE CONTENTS
• Expression vector construction
• E.coli transformation
• Protein expression conditions optimization
• Protein expression scale-up and purification
• QC analysis - STARTING MATERIAL
Protein sequence provided by customer - GUARANTEES
• High protein recovery and purity, typically >90% as assessed by SDS-PAGE analysis. Higher purity levels available upon request
• Protein concentration (BCA/Bradford method) - DELIVERABLES
2-4 mg of purified protein - TIMELINE
8 weeks
PACKAGE 2
FROM SEED STOCK TO PROTEIN
- PACKAGE CONTENTS
• Protein expression conditions optimization
• Protein expression scale-up and purification
• QC analysis - STARTING MATERIAL
Seed stock provided by the customer - GUARANTEES
• High protein recovery and purity, typically >90% as assessed by SDS-PAGE analysis. Higher purity levels available upon request.
• Protein concentration (BCA/Bradford method) - DELIVERABLES
2-4 mg of purified protein - TIMELINE
5 weeks
PACKAGE 3
FROM SOLUBLE/INSOLUBLE MATERIAL TO PROTEIN
- PACKAGE CONTENTS
• Protein purification
• QC analysis - STARTING MATERIAL
Soluble/Insoluble material provided by the customer - GUARANTEES
• High protein recovery and purity, typically >90% as assessed by SDS-PAGE analysis. Higher purity levels available upon request.
• Protein concentration (BCA/Bradford method) - DELIVERABLES
2-4 mg of purified protein - TIMELINE
3 weeks
Additional services
- Final purified protein is available with or without purification tags, and lyophilized or prepared in a customized buffer. We offer additional quality control data if needed, like: Western Blot only for tagged protein, HPLC purity analysis, and Endotoxin test.
Production process
Customer provides sequence
Expression construct generation
Trasformation and cell cultures (prokaryotes and eukaryotes)
Protein purification
QC analysis