Custom monoclonal antibody production services
We offer three different packages to produce your monoclonal antibodies
PACKAGE 1
FROM PROTEIN TO MONOCLONAL ANTIBODY
- PACKAGE CONTENTS
• Mice immunization
• Hybridoma creation and selection
• Antibody purification by antigen or protein A/G affinity chromatography
• ELISA and Western Blotting characterization - STARTING MATERIAL
Protein supplied by customer - GUARANTEES
WB and ELISA titre - DELIVERABLES
1-3 mg of purified monoclonal antibody - TIMELINE
4 months
PACKAGE 2
FROM PEPTIDE TO MONOCLONAL ANTIBODY
- PACKAGE CONTENTS
• Peptide synthesis & KLH conjugation
• Mice immunization
• Hybridoma creation and selection
• Antibody purification by antigen or protein A/G affinity chromatography
• ELISA and Western Blotting characterization - STARTING MATERIAL
Peptide sequence provided by customer (1 peptide & peptide-KLH conjugation) - GUARANTEES
WB and ELISA titre - DELIVERABLES
• 1-3 mg of purified monoclonal antibody
• 3 mg of peptide - TIMELINE
3-4 months
PACKAGE 3
FROM FULL LENGTH PROTEIN PRODUCTION TO MONOCLONAL ANTIBODY
- PACKAGE CONTENTS
• Gene synthesis and subcloning
• Protein expression and purification
• Mice immunization
• Hybridoma creation and selection
• Antibody purification by antigen or protein A/G affinity chromatography
• ELISA and Western Blotting characterization - STARTING MATERIAL
Full length protein sequence provided by customer (5 mg protein, ≥90% of purity) - GUARANTEES
WB and ELISA titre - DELIVERABLES
• 1-3 mg of recombinant antigen
• 1-3 mg of purified monoclonal antibody - TIMELINE
6 months
Additional services
- Antigen sequence analysis
- Choice of the peptides/antigens: immunoreactive epitopes analysis
- Polyclonal antibody fragmentation service: Fab, Fc and F(ab’)2 fragments
- Master cell bank of hybridoma cells: characterization and storage
- Epitope mapping: identification of the binding site of the antibody on its target
- Mycoplasma: analysis of mycoplasma strains by luminescence reaction
- Endotoxin: quantitative detection of LPS contents
Production process
Antigen design
Peptide synthesis, gene synthesis & protein production
Immunization
Immunization of mice with our optimized proprietary protocol
Cell fusion
Collection of the splenocytes and fusions with a myeloma cell line
Hybridoma selection and screening
• Hybrid cells selection (HAT selection)
• Culture supernatant screening vs. target antigen (ELISA screening)
Isolation and selection of the best monoclones
• Isolation of monoclones by limiting dilution
• Expansion and screening of the monoclones by ELISA or in target application