Custom polyclonal antibody production services
We offer three different packages to produce your polyclonal antibodies
PACKAGE 1
FROM PROTEIN TO POLYCLONAL ANTIBODY
- PACKAGE CONTENTS
• Rabbits immunization
• Serum titration
• Antibody purification by antigen or protein A/G affinity chromatography
• ELISA and Western Blotting characterization - STARTING MATERIAL
Protein supplied by customer - GUARANTEES
ELISA titre - DELIVERABLES
• 15-25 mg of purified antibodies
• Immune serum, 1 ml/rabbit
• Pre-immune serum, 1 ml rabbit - TIMELINE
2-3 months
PACKAGE 2
FROM PEPTIDE TO POLYCLONAL ANTIBODY
- PACKAGE CONTENTS
• Peptide synthesis & KLH conjugation
• Rabbits immunization
• Serum titration
• Antibody purification by antigen or protein A/G affinity chromatography
• ELISA and Western Blotting characterization - STARTING MATERIAL
Peptide sequence provided by customer (1 peptide & peptide-KLH conjugation) - GUARANTEES
ELISA titre - DELIVERABLES
• 1-3 mg of purified antibodies
• Immune serum, 1 ml/rabbit
• Pre-immune serum, 1 ml/rabbit
• 3 mg of peptide - TIMELINE
3-4 months
PACKAGE 3
FROM FULL LENGTH PROTEIN PRODUCTION TO POLYCLONAL ANTIBODY
- PACKAGE CONTENTS
• Gene synthesis and subcloning
• Protein expression and purification
• Rabbits immunization
• Serum titration
• Antibody purification by antigen or protein A/G affinity chromatography
• ELISA and Western Blotting characterization - STARTING MATERIAL
Full length protein sequence provided by customer (5 mg protein, ≥90% of purity) - GUARANTEES
ELISA titre - DELIVERABLES
• 1-3 mg of purified antibodies
• 1 mg protein, >90% of purity - TIMELINE
4-5 months
Additional services
- Antigen sequence analysis
- Choice of the peptides/antigens: immunoreactive epitopes analysis
- Polyclonal antibody fragmentation service: Fab, Fc and F(ab’)2 fragments
Production process
Antigen design
Definition of the antigen:
• Protein design
• Peptide design
Antigen production
Protein production in an expression system of your choice
Immunization
Immunization of rabbits with our optimized proprietary protocol
Antibody testing
Titration by ELISA
Serum purification and antibody characterization
• Purification against protein A or protein G
• Purification against antigen
• Antibody characterization