DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Dinophysis spp PCR detection Kit 50

Dinophysis spp. PCR detection Kit allows the detection of Dinophysis DNA using polymerase chain reaction (PCR). PCR primers specifically detect all the Dinophysis species. The kit contains reagents and enzymes for the specific amplification of a 366 bp region of the Dinophysis genome. This PCR assay is able to detect down to one cell per reaction. To check for possible PCR inhibition, an internal control, giving an amplicon of 214 bp, is also supplied.

Kit contents:

- 2 x 1ml Dinophysis spp. Mix - containing primers, dNTPs, 10X reaction buffer, MgCl2 and an internal control plasmid DNA including specific primers.
- 1 x 0,5ml Dinophysis Positive Control - DNA containing D.sacculus specific sequence
- 1 x 10 μl Hot-Rescue DNA Polymerase
- 1 x 1ml PCR-grade Water.


 

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Identification of Dinophysis spp. DNA by polymerase chain reaction (PCR).

Diarrhoeic shellfish poisoning (DSP) is a syndrome predominantly caused by the consumption of contaminated shellfish. DSP toxins are produced by dinoflagellates such as Dinophysis spp. Coastal water monitoring programmed to detect the presence of toxic algae could provide an essential tool to access bloom formation and consequently potential shellfish contamination. PCR-based methods are attractive tools for coastal water monitoring programmes because they can rapidly detect as low as one cell of specific organism in mixed ecological scenarios. These methods have been successfully applied to seawater samples for the identification of several dinoflagellates, reducing the time for the standard monitoring procedures (microscope observation).

 

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