DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Salmonella spp. FLUO kit (50 tests)

The Salmonella spp. FLUO kit provides an easy-to-use mastermix dedicated for the use with the instruments with two-plex capability. The kit contains reagent, enzyme and positive control for the successful amplification and detection of DNA from Salmonella spp. in multiplex Real-Time PCR using dual-labelled probes. Up to 2 genes (1 control gene and 1 target gene) can be detected simultaneously in the same reaction.


Kit contents

- 2 x 0.5 ml 1X master mix
- 1 x 15 μl 5U/ μl DNA polymerase
- 1 x 0.2 ml positive control (105cells for the target specie/5μl)
- 1 x 1ml PCR grade water


 

 

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Info

SUGGESTED COMPANION PRODUCTS:

#MBK0061 Fast DNA extraction Kit

#MBK0063 Bacterial DNA isolation Single Step 

 

The kit could be used for the identification of Salmonella spp. by multiplex Real-time PCR.

Intoxications and infections caused by food-borne pathogens represent an increasing public health problem, with nearly a quarter of the population at higher risk for illness today (Oliver et al., 2005). Several outbreaks of food-borne illnesses following consumption of several food caused by Salmonella spp. have been reported in recent years, indicating the importance of this problem in safeguarding public health. The fast and accurate identification of Salmonella spp. from food samples by Public health agencies and diagnostic laboratories insures not only a better quality of products, but also the possibility to adopt timely precautionary measures to limit the spread of infection in case of an outbreak. Conventional assays in common use can take up to days or more. The Salmonella spp. FLUO kit represents an alternative PCR-based approach for the qualitative detection of this pathogen. The kit uses DNA primer and fluorescent probe specific for the target organism. If pathogen is present, DNA is amplified and the increased fluorescence signals are recordered in Real-Time. The internal control, present in the amplification mix, assesses the efficiency of amplification reaction by checking the presence of inhibitory factors and ensuring reliability of negative results.

Available four specific formulations optimized for different Real-Time PCR instruments:

PN# MBK0054a for ABI Prism® 7000, 7700, 7900HT SDS, GeneAMP5700

PN# MBK0054b for ABI Prism® 7300, 7500, SDS

PN# MBK0054c for Bio-Rad/MJ Research, Cephied, Corbett Research, Eppendorf and other instruments that do not require ROX dye

PN# MBK0054d for Step One - Applied Biosystems

 

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