DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Multipathogen FLUO kit (S+L)

L.monocytogenes and Salmonella spp. kit provides an easy-to-use mastermix dedicated for the use with the instruments with three-plex capability. The kit contains reagent, enzyme and positive control for the successful amplification and detection of DNA from L. monocytogenes and Salmonella spp. in multiplex Real-Time PCR using dual-labelled probes. Up to 3 genes (1 control gene and 2 target genes) can be detected simultaneously in the same reaction.


Kit contents:


- 2 x 0.5 ml 1X master mix
- 1 x 15 μl 5U/ μl DNA polymerase
- 1 x 0.2 ml positive control (105 cells for the target species/5 μl)
- 1 x 1 ml PCR grade water



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The kit could be used for the identification of L.monocytogenes and Salmonella spp. by multiplex Real-time PCR.

 

Intoxications and infections caused by food-borne pathogens represent an increasing public health problem, with nearly a quarter of the population at higher risk for illness today (Oliver et al., 2005). Several outbreaks of food-borne illnesses following consumption of several food caused by L. monocytogenes and Salmonella spp. have been reported in recent years, indicating the importance of this problem in safeguarding public health. The fast and accurate identification of these pathogens from food samples by Public health agencies and diagnostic laboratories insures not only a better quality of products, but also the possibility to adopt timely precautionary measures to limit the spread of infection in case of an outbreak. Conventional assays in common use can take up to days or more.

L. monocytogenes and Salmonella spp. FLUO kit represents an alternative PCR-based approach for the qualitative detection of this pathogen. The kit uses DNA primer and fluorescent probe specific for the target organism. If pathogen is present, DNA is amplified and the increased fluorescence signals are recordered in Real-Time. The internal control, present in the amplification mix, assesses the efficiency of amplification reaction by checking the presence of inhibitory factors and ensuring reliability of negative results. A multiplex assay with two dyes is used: probes for the target DNA and the internal control, each labeled with different fluorophores are in the same tube. Results are obtained within a few hours following an enrichment step and subsequent DNA extraction.

 

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