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Photobacterium damselae PCR Detection Kit

Photobacterium Damselae PCR detection Kit allows the detection of DNA from P. damselae using Polymerase Chain Reaction (PCR). PCR primer combination specifically discriminates both subspecies piscicida and damselae. The kit contains reagents and enzymes for the specific amplification of two target sequences: a 448 bp region specific for the subsp. damselae and a 297 bp region present in the genome of all Photobacterium damselae subsp. piscicida. Few P. damselae subsp. damselae isolates, showing the 297 bp amplicon, can be identified by the presence of the 448 bp product. To reveal possible PCR inhibition, an internal control, giving an amplicon of 112 bp, is also supplied in the PCR mix.


Kit contents:


- 2 x 1250μl Photobacterium damselae Mix
- 1 x 15μl Hot-Rescue DNA Polymerase (5U/μl)
- 1 x 15μl Photobacterium damselae Positive Control
- 1 x 1000μl PCR-Grade Water


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Info

Identification of Photobacterium damselae DNA and discrimination between subspecies damselae and piscicida by polymerase chain reaction (PCR).

Photobacterium damselae subsp. piscicida is the causative agent of fish pasteurellosis (or pseudotuberculosis), a bacterial septicemia described in a wide variety of marine fish. The disease has great economic impact in aquaculture of the Mediterranean area and Japan (1). Current identification methods rely on culture isolation followed by serological and biochemical tests. However, microbiological diagnosis can be hampered by the slow growth of this species, which can be easily obscured by other fast growing bacteria (2), or by the presence of viable but nonculturable cells (3). Moreover, phenotypic characterization revealed a considerable degree of variability within this subspecies and P. damselae subsp. damselae (4), (5). PCR-based detection systems are a good alternative to microbiological standard methods, offering sensitivity and shortened analysis time, but their application to P. damselae subsp. piscicida identification has been limited by the high degree of DNA base sequence similarity between the two subspecies piscicida and damselae (6).

 

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