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Magazine / Pbrt

The PBRT-KIT identified new replicons which were not detected by the 2005 PBRT scheme

Comparative analysis of the standard PCR-Based Replicon Typing (PBRT) with the commercial PBRT-KIT

Carloni et al., Plasmid In press

 

ECDC/EFSA/EMA first joint report on the integrated analysis of the consumption of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from humans and food-producing animals

The ECDC, the EFSA and the EMA have for the first time jointly explored associations between consumption of antimicrobials in humans and food-producing animals, and antimicrobial resistance in bacteria from humans and food-producing animals, using 2011 and 2012 data currently available from their relevant five EU monitoring networks.
Read more http://ecdc.europa.eu/en/publications/Publications/antimicrobial-resistance-JIACRA-report.pdf

 

 

EMA acts upon request from European Commission following detection of colistin-resistant bacteria

EMA acts upon request from European Commission following detection of colistin-resistant bacteria

The European Medicines Agency (EMA) has received a request from the European Commission to update its advice on the use in animals of colistin, which is one of the last-resort antibiotics to treat certain bacterial infections in humans. Read more

PBRT KIT can detect the replicon of the IncI2 plasmid recently associated with the colistin resistance mcr-1 gene

PBRT KIT can detect the replicon of the IncI2 plasmid recently associated with the colistin resistance mcr-1 gene Liu et al., Lancet 2015

 

IncL and IncM plasmids constitute two separate interspecies vehicle for the dissemination of resistance genes

The PBRT method has been updated! Increased specificity and sensitivity in the detection and discimination of the IncL and IncM plasmids that are now differentiated in two distinct groups.

 

Differentiation of IncL and IncM Plasmids Associated with the Spread of Clinically Relevant Antimicrobial Resistance. Carattoli A. et al.PLoS One 2015

Characterization of the CTX-M-15-encoding plasmid in a Klebsiella pneumoniae ST17 strain responsible for an outbreak at a Norwegian neonatal intensive care unit

Persistence of a pKPN3-Like CTX-M-15-Encoding IncFIIK Plasmid in a Klebsiella pneumonia ST17 Host during Two Years of Intestinal Colonization. Löhr IH et al., PLoS One 2015 

SCIENTIFIC REPORT OF ECDC, EFSA AND EMA

The European Centre for Disease Prevention and Control, the European Food Safety Authority and the European Medicines Agency published for the first time an integrated report about the possible relationships between the consumption of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from humans and food-producing animals in Europe. For the full report click here.

 

The PBRT BLOG

PBRT KIT is a PCR-based assay that allows fast and easy plasmid characterization and molecular identification through replicon detection. The system initially developed and validated by Dr. Alessandra Carattoli and subsequently industrialized by Diatheva is the only kit available on the market and starting from 2011 is used in many laboratories in various country.

Now Diatheva introduces a new space entirely dedicated to PCR Based Replicon Typing and its users: The PBRT BLOG that will be continuously updated with news about PBRT kit and much more. New publications and applications but also conferences, updates from congresses and work in progress will be disclosed.

This BLOG would be a meeting point to exchange experiences, opinions and all that will be helpful for you! For more news, follow us!

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