The HIV-1 DNA Test PRO is intended for the qualitative and quantitative detection of HIV-1 DNA using real-time PCR that is to date the most sensitive and reproducible technology for the monitoring of HIV-1 persistence in infected cells. HIV-1 DNA Test PRO allows the simultaneous amplification of HIV DNA and human Telomerase Reverse Transcriptase (hTERT). The endogenous gene allows to verify DNA extraction success, to determine the presence of PCR inhibitors and to obtain HIV DNA copies without cellular DNA quantification. The HIV DNA provides an overall quantification of all viral forms of HIV-1 DNA: stable integrated proviruses and extrachromosomal forms, including both circular (1-LTR and 2-LTR) and linear forms, each playing a different role in HIV replication and pathophysiology. The evaluation of this marker at different stages in the history of infection can help clinicians to choose the best therapeutic strategies and to control the progression of HIV infection. Moreover HIV-1 proviral DNA detection is indicated for HIV diagnosis in infants borni to infected mothers below 2-years of age: serology may remain positive due to passive transfer of antibodies through placenta. The detection of proviral DNA is a sensitive and specific way of diagnosing HIV infection and is a very useful adjunct test in the context of primary infection in those with negative or indeterminate serology.