Hot Rescue Real-Time PCR Kit – SYBR is a 2X premix reagent designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The provided master mixes contain buffer, dNTPs, thermostable Hot Rescue PLUS DNA polymerase and SYBR Green dye.
Hot Rescue Real-Time PCR Kit – SYBR
Description
Hot Rescue real-time PCR Kit – SYBR includes Hot Rescue PLUS DNA Polymerase, a chemically modified Hot-start DNA polymerase which has no activity below 74 °C and requires a thermal activation of 10 minutes at 95 °C to reach maximal activity, thus avoiding non-specific priming at low temperature during the PCR set-up. Hot Rescue Real Time PCR Kit – SYBR is particularly recommended for PCR applications that require ultra low levels of DNA and for amplification of target which do not allow the design of labelled probes.
Additional information
| Size | 400 tests |
|---|---|
| Application | Life Sciences |
| Product type | Reagents for research |
| Reagent type | PCR |
| Category | Molecular Biology |
Technical information
| Size | 400 tests |
|---|---|
| Technology | Real-time PCR |
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References
| Real-time PCR to differentiate among Leishmania (Viannia) subgenus, Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis: Application on Brazilian clinical samples | PUBMED ID: 31606374 |
