Hot Rescue Real-Time PCR Kit – SYBR is a 2X premix reagent designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The provided master mixes contain buffer, dNTPs, thermostable Hot Rescue PLUS DNA polymerase and SYBR Green dye.
Hot Rescue Real-Time PCR Kit – SYBR
Hot Rescue real-time PCR Kit – SYBR includes Hot Rescue PLUS DNA Polymerase, a chemically modified Hot-start DNA polymerase which has no activity below 74 °C and requires a thermal activation of 10 minutes at 95 °C to reach maximal activity, thus avoiding non-specific priming at low temperature during the PCR set-up. Hot Rescue Real Time PCR Kit – SYBR is particularly recommended for PCR applications that require ultra low levels of DNA and for amplification of target which do not allow the design of labelled probes.
|Product type||Reagents for research|
|Real-time PCR to differentiate among Leishmania (Viannia) subgenus, Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis: Application on Brazilian clinical samples||PUBMED ID: 31606374|