Hot Rescue Real-Time PCR Kit – SYBR is a 2X premix reagent designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The provided master mixes contain buffer, dNTPs, thermostable Hot Rescue PLUS DNA polymerase and SYBR Green dye.
Hot Rescue Real-Time PCR Kit – SYBR
Description
Hot Rescue real-time PCR Kit – SYBR includes Hot Rescue PLUS DNA Polymerase, a chemically modified Hot-start DNA polymerase which has no activity below 74 °C and requires a thermal activation of 10 minutes at 95 °C to reach maximal activity, thus avoiding non-specific priming at low temperature during the PCR set-up. Hot Rescue Real Time PCR Kit – SYBR is particularly recommended for PCR applications that require ultra low levels of DNA and for amplification of target which do not allow the design of labelled probes.
Additional information
Size | 400 tests |
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Application | Life Sciences |
Product type | Reagents for research |
Reagent type | PCR |
Category | Molecular Biology |
Technical information
Size | 400 tests |
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Technology | Real-time PCR |
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References
Real-time PCR to differentiate among Leishmania (Viannia) subgenus, Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis: Application on Brazilian clinical samples | PUBMED ID: 31606374 |