The FAST Avian Sexing PCR Kit is a molecular method based on the examination of sexual chromosomes starting from samples of feathers.
FAST Avian Sexing PCR Kit
Gender identification of species is very important for veterinary, medical, and ecological sciences. Nevertheless, sexing based on external morphology is often impossible, because more than 50% of bird species are monomorphic (Vucicevic et al., 2012). Traditional methods for sexual identification as the observation of sex-specific behaviour, laparoscopy, laparotomy or cloacal examination are time consuming, expensive, and, in some case, invasive and dangerous. To avoid this limitation, molecular sexing-based methods using non-invasive means, such as feathers, were improved (Morinha et al., 2012).
The most common method for avian sexing is based on amplification and electrophoresis of the Chromo-helicase-DNA binding protein (CHD) gene on the W and Z chromosomes (Griffiths et al., 2008). The detection of these genes allows for sexing of unknown samples.The FAST Avian Sexing PCR Kit is a molecular method based on the examination of sexual chromosomes starting from samples of feathers.
The FAST Avian Sexing PCR Kit allows to analyze birds DNA starting from samples of feathers by Polymerase Chain reaction (PCR) using a ready to use PCR mix containing specific primers pair for amplification of CHD W and CHD-Z genes.
|Product type||Diagnostic kit|
|Technology||PCR end point|
|Testing time||85 min|
|Target gene||CHD-W and CHD-Z genes|