2X PCR Master Mix kit is an optimized 2X premix reagent containing PCR Reaction Buffer, MgCl2 and dNTP, designed for quick and easy assembly (via dilution only) of the PCR mix.
2X PCR Master Mix Kit
The kit includes Hot Rescue DNA Polymerase, which is able to amplify PCR products with one A at the 3′-termini that may be directly cloned into a T-vector with >80% cloning efficiency. It’s also possible to clone the PCR product in blunt-end vectors after blunting and phosphorylation of the end. Hot Rescue DNA Polymerase has an efficient proofreading activity allowing a higher fidelity than any other Taq, therefore 2X PCR Master Mix can be successfully used for PCR which require high-fidelity.
|Product type||Reagents for research|